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inhibiting pro-inflammatory cytokines such as IL-6, TNF-α, and matrix metalloproteinases (MMPs)
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through NF-κB pathway suppression . Additionally, diclofenac (Dic), a NSAID is commonly
administered topically or orally to alleviate OA pain and inflammation by inhibiting
45
cyclooxygenase (COX) activity . Therefore, in this study, Cur, Dex and Dic were employed as
drug models for proof-of-concept to investigate the drug screening efficacy of the constructed
osteochondral unit.
Figure 1. Schematic illustrating the preparation of the hierarchical osteochondral architectures
based on the PLGA PMs using the combinatorial microfluidics and bioprinting technologies for
drug screening.
2. Experimental Section
2.1. Materials
Polyvinyl alcohol (PVA), PLGA (lactide:glycolide = 75:25, 66-107 kDa), and gelatin were
obtained from Sigma-Aldrich (St. Louis, USA). Cur (99.7%) and dichloromethane (DCM) were
purchased from Sinopharm Group Chemical Reagent Co. Ltd. (China). Dic, Dex, Ascorbic acid
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