Page 83 - GTM-2-3

Global Translational Medicine

ORIGINAL RESEARCH ARTICLE
A simple, fast, and cost-effective high-

performance liquid chromatography-ultraviolet
validated method to quantify lacosamide in

therapeutic drug monitoring

1
2
1
Ángeles Gloria Rodríguez-Basso , María Cecilia Kravetz *, María Sylvia Viola ,
3
2
Florencia Ayelén Fernández , Nicolás Martín Barrionuevo , Damián Consalvo ,
2
2
Mariano Núñez , and Guillermo Federico Bramuglia 1,4
1 Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires,
Buenos Aires, Argentina
2 Institute of Pharmacology, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
3 Institute of Neurology and Neurosurgery, Sanatorio de Los Arcos, Buenos Aires, Argentina
4 Fundación Investigar, Buenos Aires, Argentina

Abstract
Lacosamide is a third-generation anticonvulsant used in the treatment of epilepsy. While
therapeutic levels for various patient subpopulations are still under investigation to
achieve optimal responses, therapeutic drug monitoring has been proven to be useful
in improving patient management. To ensure the reliability of results, it is essential to
*Corresponding author: establish a reliable quantitative method. Thus, the aim of this study was to develop and
María Cecilia Kravetz
(mariaceciliakravetz@gmail.com) validate a simple and feasible method for quantifying lacosamide in human plasma,
with the specific aim of facilitating drug monitoring purposes. A high-performance
Citation: Rodríguez-Basso ÁG,
Kravetz MC, Viola MS, et al., liquid chromatography method with ultraviolet detection was developed for the
2023, A simple, fast, and quantification of lacosamide in human plasma. Analyte recovery was achieved through
cost-effective high-performance ethyl acetate extraction, with propranolol serving as the internal standard. A C18
liquid chromatography-ultraviolet
validated method to quantify column and a mobile phase consisting of 10 mM phosphate buffer and acetonitrile
lacosamide in therapeutic drug (70:30 v/v, pH = 3.5) were employed. Validation parameters included specificity, linearity,
monitoring. Global Transl Med, 2(3): repeatability, precision, accuracy, sensitivity, and stability. The method exhibited linearity
1265. within the range of 2.5 – 30 µg/ml (R = 0.997), with a limit of quantitation of 2.29 µg/ml.
2
https://doi.org/10.36922/gtm.1265
The average recovery percentage was 100.2%, and it proved to be accurate, precise, and
Received: July 7, 2023 specific. In plasma samples, the drug content remained stable for 72 h at 4°C, 1 month
Accepted: September 5, 2023
Published Online: September 26, at −20°C, and 2 years at −80°C. The post-processed sample remained stable for 1 week
2023 under all tested conditions. Due to its simplicity, short analysis time, sensitivity, and
Copyright: © 2023 Author(s). cost-effectiveness, the proposed analytical method proves to be useful for therapeutic
This is an Open-Access article monitoring and pharmacokinetic studies of lacosamide.
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, Keywords: High-performance liquid chromatography; Lacosamide; Pharmacokinetics
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Lacosamide is a third-generation antiepileptic drug (AED) used to treat epileptic seizures,
affiliations. both as monotherapy and in combination with other AEDs. Its primary mechanism of

Volume 2 Issue 3 (2023) 1 https://doi.org/10.36922/gtm.1265