Page 42 - IJB-9-5

International Journal of Bioprinting

RESEARCH ARTICLE
Swelling compensation of engineered

vasculature fabricated by additive manufacturing
and sacrifice-based technique using

thermoresponsive hydrogel

Xue Yang , Shuai Li *, Xin Sun , Ya Ren , Lei Qiang , Yihao Liu , Jinwu Wang *,
3
3,5
4
1
1†
2†
3†
Kerong Dai *
1,3
1 College of Medicine, Southwest Jiaotong University, No. 144 Jiaoda Road, Chengdu 610031,
People’s Republic of China
2 Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine,
No. 79 Qingchun Rd, Hangzhou 310003, People’s Republic of China
3
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai
Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Rd,
Shanghai 200011, People’s Republic of China
4 School of Materials Science and Engineering, Southwest Jiaotong University, No. 111 2nd Ring Rd,
Chengdu 611756, People’s Republic of China
5
Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, No.
1956 Huashan Rd, Shanghai 200030, People’s Republic of China
† These authors contributed equally
to this work.
*Corresponding authors:
Shuai Li (allenle1991@sina.com) Abstract
Jinwu Wang
(wangjw-team@shsmu.edu.cn) Engineered vasculature is widely employed to maintain the cell viability within
Kerong Dai in vitro tissues. A variety of fabrication techniques for engineered vasculature have
(krdai@163.com) been explored, with combination of additive manufacturing with a sacrifice-based
Citation: Yang X, Li S, Sun X, et al., technique being the most common approach. However, the size deformation of
2023, Swelling compensation of vasculature caused by the swelling of sacrificial materials remains unaddressed. In
engineered vasculature fabricated
by additive manufacturing and this study, Pluronic F-127 (PF-127), the most widely used sacrificial material, was
sacrifice-based technique using employed to study the deformation of the vasculature. Then, a thermoresponsive
thermoresponsive hydrogel. Int J hydrogel comprising poly(N-isopropylacrylamide) (PNIPAM) and gelatin methacrylate
Bioprint, 9(5): 749.
https://doi.org/10.18063/ijb.749 (GelMA) was used to induce volume shrinkage at 37°C to compensate for the
deformation of vasculature caused by the swelling of a three-dimensional (3D)-
Received: January 31, 2023 printed sacrificial template, and to generate vasculature of a smaller size than that
Accepted: March 2, 2023
Published Online: May 10, 2023 after deformation. Our results showed that the vasculature diameter increased after
the sacrificial template was removed, whereas it decreased to the designed diameter
Copyright: © 2023 Author(s).
This is an Open Access article after the volume shrinkage. Human umbilical vein endothelial cells (HUVECs)
distributed under the terms of the formed an endothelial monolayer in the engineered vasculature. Osteosarcoma
Creative Commons Attribution cells (OCs) were loaded into a hierarchical vasculature within the thermoresponsive
License, permitting distribution,
and reproduction in any medium, hydrogel to investigate the interaction between HUVECs and OCs. New blood vessel
provided the original work is infiltration was observed within the lumen of the engineered vasculature after
properly cited. in vivo subcutaneous implantation for 4 weeks. In addition, engineered vasculature
Publisher’s Note: Whioce was implanted in a rat ischemia model to further study the function of engineered
Publishing remains neutral with vasculature for blood vessel infiltration. This study presents a small method aiming to
regard to jurisdictional claims in
published maps and institutional accurately create engineered vasculature by additive manufacturing and a sacrifice-
affiliations. based technique.

Volume 9 Issue 5 (2023) 34 https://doi.org/10.18063/ijb.749