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Microbes & Immunity Establishment of a novel anti-human CCR8 monoclonal antibody

A A

B
B

C
C

Figure 4. The analysis of the binding affinity of anti-hCCR8 monoclonal
antibodies for CHO/hCCR8. CHO/hCCR8 cells were suspended in 100
µL of serially diluted C Mab-21 (100 µg/mL – 0.006 µg/mL) (A), S19017D Figure 5. The analysis of the binding affinity of anti-hCCR8 monoclonal
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(10 µg/mL – 0.0006 µg/mL) (B), or L263G8 (10 µg/mL – 0.0006 µg/ antibodies for TALL-1. TALL-1 cells were suspended in 100 µL of serially
mL) (C). The cells were then treated with Alexa Fluor 488-conjugated diluted C Mab-21 (100 µg/mL – 0.006 µg/mL) (A), S19017D (10 µg/
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anti-mouse immunoglobulin G. Fluorescence data were subsequently mL, 2.5 µg/mL – 0.0006 µg/mL) (B), or L263G8 (0.625 µg/mL – 0.0006
collected using the BD FACSLyric, and the EC values were calculated µg/mL) (C). Then, cells were treated with Alexa Fluor 488-conjugated
50
by GraphPad PRISM 6. anti-mouse immunoglobulin G. Fluorescence data were subsequently
Abbreviations: GeoMean: Geometric mean; hCCR8: Human C-C motif collected using the BD FACSLyric, following the calculation of the EC 50
chemokine receptor-8. by GraphPad PRISM 6.
Abbreviations: GeoMean: Geometric mean; hCCR8: Human C-C motif
chemokine receptor-8.
the results of omics analysis. CCR8 is suggested to be
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involved in the pathogenesis of various cancer types. 24,25 progression and treatment resistance. 58,59 Recently, the
CAFs, similar to Tregs, are one of the tumor-suppressive phenotypes of CAF have been reported to associate with
factors known to interfere with the function of tumor either better or worse outcomes in NSCLC patients.
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immune cells by promoting fibrosis and constructing Although further functional analysis of CCR8-expressing
the extracellular matrix in the TME. CAFs with a CAFs is required, targeting CCR8 could suppress Treg and
1,57
myofibroblastic-like phenotype transfer large amounts CAFs, and lead to synergistic antitumor immunotherapy
of proteins to the surrounding endothelial cells through results. Therefore, it is well worth evaluating the impact of
matrix-bound vesicles, which may contribute to cancer C Mab-21 on CAFs.
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Volume 2 Issue 2 (2025) 132 doi: 10.36922/mi.4661