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Advanced Neurology

ORIGINAL RESEARCH ARTICLE
Distinct behavioral effects of short-term

voluntary running in phosphatase and tensin
homolog deleted on chromosome 10 neuronal

haploinsufficient mice

1
1
Diana Zukas Andreotti , Natália Prudente de Mello , Amanda Galvão Paixão ,
1
1,2
2
1
Cristoforo Scavone , Ana Maria Orellana , and Elisa Mitiko Kawamoto *
1 Laboratory of Molecular and Functional Neurobiology, Department of Pharmacology, Institute of
Biomedical Sciences, University of São Paulo, São Paulo, Brazil
2 Laboratory of Molecular Neuropharmacology, Department of Pharmacology, Institute of Biomedical
Sciences, University of São Paulo, São Paulo, Brazil
Abstract

Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a
tumor suppressor with functions related to its phosphatase activity. PTEN plays
various roles, such as cell proliferation, survival, and migration and is involved
in neurogenesis and synaptic plasticity in the central nervous system. It has been
reported that running could have protective effects against the aging process
and neurodegenerative diseases. Therefore, we aimed to evaluate the effects of
physical exercise on behavioral and biochemical aspects of PTEN-conditioned
*Corresponding author:
Elisa Mitiko Kawamoto knockout female mice. We observed that 10 days of voluntary running positively
(elisamk@usp.br) affected fear memory but caused no changes in anxiety-like behavior. However, it
Citation: Andreotti DZ, de Mello NP, was unable to counteract the social recognition memory deficit in PTEN neuronal
Paixão AG, et al., 2023, Distinct haploinsufficient mice. In terms of biochemical aspects, we observed that short-
behavioral effects of short-term term running reduced S6 phosphorylation in PTEN heterozygous mice and PTEN
voluntary running in phosphatase
and tensin homolog deleted protein expression independent of the genotype. In addition, PTEN heterozygous
on chromosome 10 neuronal mice presented reduced N-methyl-D-aspartate subunit NR1 protein expression. Our
haploinsufficient mice. Adv Neuro, results regarding decreased S6 phosphorylation in HT mice suggest that short-term
2(3): 0872.
https://doi.org/10.36922/an.0872 voluntary running could have induced a protective effect in reducing dysregulated
cell growth, possibly related to the downregulation of tumor suppressor expression/
Received: April 27, 2023 activity such as PTEN. Moreover, running induced distinct behavioral effects in PTEN-
Accepted: July 10, 2023 conditioned knockout mice.
Published Online: August 2, 2023
Copyright: © 2023 Author(s). Keywords: Memory; Brain; Anxiety; Plasticity; Sociability; Running
This is an Open-Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium, 1. Introduction
provided the original work is
properly cited. Originally known as a tumor suppressor protein, phosphatase and tensin homolog
Publisher’s Note: AccScience deleted on chromosome 10 (PTEN) [1,2] is a dual phosphatase with both protein and
Publishing remains neutral with lipid phosphatase activities regulating several cellular functions. Through its lipid
regard to jurisdictional claims in
published maps and institutional phosphatase motif, PTEN dephosphorylates 3,4,5-phosphatidylinositol triphosphate
affiliations. (PIP ), converting it into 4,5-phosphatidylinositol bisphosphate (PIP ), which negatively
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Volume 2 Issue 3 (2023) 1 https://doi.org/10.36922/an.0872