Page 88 - BH-2-4

Brain & Heart

ORIGINAL RESEARCH ARTICLE
Human dental pulp MSCs attenuated motor

neuron dysfunction and prolonged lifespan in
ALS murine model

3
Shihe Jiang 1† , Xiuchen Guan 2† , Meng Shi , Ying Zhang 1 , Xindi Li 1 ,
4
5
Yingying Su 3 , Hao Wang , Jian Zhou , Fu-Dong Shi 1 , Songling Wang ,
3
1
and Wei-Na Jin *
1 China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital,
Capital Medical University, Beijing, China
2 Department of Orthodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical
University, Beijing, China
3 Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
4 Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key
Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical
University, Beijing, China
5 Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function
Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Capital
Medical University, Beijing, China

† These authors contributed equally Abstract
to this work.
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that
*Corresponding author:
Wei-Na Jin causes skeletal muscle weakness and atrophy, resulting in respiratory failure and a
(weina.jin@ncrcnd.org.cn) short lifespan. Considering the lack of effective treatment, this study investigated
Citation: Jiang S, Guan X, the effects of human dental pulp stem cells (hDPSCs) on the clinical symptoms and
Shi M, et al. Human dental pulp potential mechanisms in a mouse model of ALS superoxide dismutase 1 (SOD1-
MSCs attenuated motor neuron G93A). Neurological assessments, including neurological scoring, rotarod testing,
dysfunction and prolonged lifespan
in ALS murine model. Brain & Heart. and 7-T magnetic resonance imaging, were conducted to evaluate neurological
2024;2(4):3996. impairments. Survival rates and body weight of the mice were also recorded.
doi: 10.36922/bh.3996 Immunofluorescence staining and flow cytometry analyses were performed to
Received: June 20, 2024 investigate the number of neurons and infiltrated inflammatory cells in the spinal
cord as well as the central nervous system. The results indicate that infusion of
Accepted: September 4, 2024
hDPSCs increased the body weight, mitigated motor neuron dysfunction, and
Published Online: October 10, extended the lifespan of SOD1-G93A mice by approximately 15 days. Moreover,
2024 hDPSCs infusion reduced the degree of spinal cord atrophy. Results suggested
Copyright: © 2024 Author(s). that the number of neurons in the central nervous system of SOD1-G93A mice
This is an Open-Access article was significantly decreased, but hDPSCs infusion resulted in an increase in
distributed under the terms of the
Creative Commons Attribution these numbers. However, hDPSCs infusion had no obvious effect on microglia
License, permitting distribution, phenotypes in SOD1-G93A mice. This study emphasizes the potential of hDPSCs to
and reproduction in any medium, mitigate neuronal loss in an ALS mouse model, suggesting a promising therapeutic
provided the original work is
properly cited. avenue for ALS.
Publisher’s Note: AccScience
Publishing remains neutral with Keywords: Human dental pulp stem cells; Amyotrophic lateral sclerosis; Neurons;
regard to jurisdictional claims in
published maps and institutional Inflammation; Murine model
affiliations.

Volume 2 Issue 4 (2024) 1 doi: 10.36922/bh.3996