Page 269 - EJMO-9-2

Eurasian Journal of Medicine

and Oncology

ORIGINAL RESEARCH ARTICLE
Increased angiopoietin-like protein 8 expression

in colorectal carcinoma patients with liver
metastasis

Hendra Susanto * , Adeodatus Yuda Handaya 2 , Arum Linangkung 2 ,
1
Wira Eka Putra 1 , Zulqarnain Mohamed 3 , Jerry Dwi Trijoyo Purnomo 4 ,
Moch Sholeh 5 , and Sa’diyatul Rizqie Amaliyah Firdaus 5

1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang,
Malang, East Java, Indonesia
2 Digestive Surgery Division, Faculty of Medicine, Universitas Gadjah Mada, Sleman, Yogyakarta,
Indonesia
3 Department of Biological Sciences, Institute of Biological Sciences, Faculty of Science, University
of Malaya, Kuala Lumpur, Malaysia
4 Department of Statistics, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh
Nopember, Surabaya, East Java, Indonesia
5 Department of Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, East Java
Indonesia

*Corresponding author:
Hendra Susanto
(hendrabio@um.ac.id)
Citation: Susanto H, Handaya AY, Abstract
Linangkung A, et al. Increased
angiopoietin-like protein 8 Colorectal cancer (CRC) is the third most common cancer in men and the second
expression in colorectal carcinoma most common in women, with a 5-year survival rate. The disease burden of CRC
patients with liver metastasis. can be exacerbated by a sedentary lifestyle, lack of physical exercise, smoking, and
Eurasian J Med Oncol.
2025;9(2):261-269. obesity. Angiopoietin-like protein 8 (ANGPTL8), a liver hormone, plays a crucial
doi: 10.36922/EJMO025080034 role in lipid and glucose metabolism and is also implicated in the development
Received: February 18, 2025 of certain cancers. However, the basic profile and activity of this hormone in CRC
with liver metastasis remains unknown. This study aims to establish the baseline
1st revised: March 24, 2025 expression of ANGPTL8 in tumors of CRC patients with and without liver metastasis.
2nd revised: April 2, 2025 After histopathological screening and computed tomography scans, 41 patients
3rd revised: April 22, 2025 were identified and included in this study, consisting of 20 CRC patients without
liver metastasis and 21 with liver metastasis. Tumor samples from each group
4th revised: April 23, 2025 were compared to their respective adjacent normal tissues. Quantitative real-
Accepted: April 24, 2025 time polymerase chain reaction and enzyme-linked immunosorbent assay were
Published online: May 9, 2025 employed to assess the ANGPTL8 expression at both mRNA and protein levels. The
results show that ANGPTL8 expression is higher in tumors of CRC patients with
Copyright: © 2025 Author(s). liver metastasis compared to those without liver metastasis. ANGPTL8 expression
This is an Open-Access article
distributed under the terms of the is significantly correlated with body mass index (BMI) (Pearson’s correlation
Creative Commons Attribution coefficient = −0.367, p≤0.05) and could serve as a significant predictor of BMI
License, permitting distribution, (p≤0.05). These findings suggest that ANGPTL8 may enhance tumor development
and reproduction in any medium,
provided the original work is in CRC patients and is associated with body weight changes, contributing to weight
properly cited. loss symptoms during metastasis.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in Keywords: ANGPTL8; Body weight loss; Colorectal cancer; Liver metastasis
published maps and institutional
affiliations.

Volume 9 Issue 2 (2025) 261 doi: 10.36922/EJMO025080034