Page 9 - GPD-1-1

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Structural, functional, phylogenetic, and molecular

dynamic simulation study of PEST-containing
nuclear protein: An e-science view

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Nazeer Hussain Khan , Muhammad Shahid , Wenkang Wang ,
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Saadullah Khatak , Ebenezeri Erasto Ngowi , Salma S. Mahmoud ,
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1,5
1,2
Hao-Jie Chen , Lei Qian ,Yangzhe Qin , Tao Li , Muhammad Zubair ,
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Shazrul Fazry , Dong-Dong Wu *, Chun Yang Zhang 9,10 *, and Xin-Ying Ji 1,11 *
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1 Henan International Joint Laboratory for Nuclear Protein Regulation, School of Basic Medical
Sciences, Henan University, Kaifeng, Henan, China
2 School of Life Sciences, Henan University, Kaifeng, Henan, China
3 Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti
Kebangsaan Malaysia, Bangi, Selangor, Malaysia
4 Department of Breast Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou,
Henan, China
5 Department of Biological Sciences, Faculty of Science, Dar es Salaam University College of
Education, Dar es Salaam, Tanzania
6 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams
University, Cairo, Egypt
7 Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Pattoki Campus,
Pakistan
8 School of Stomatology, Henan University, Kaifeng, Henan, China
*Corresponding authors: 9 Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou,
Dong-Dong Wu
(ddwubiomed2010@163.com) Henan, P.R. China
Chun Yang Zhang 10 Department of General Thoracic Surgery, Hami Central Hospital, Hami, Xinjiang, P.R.
(zcy198200@163.com) China - Institute of Nursing and Health, Henan University, Kaifeng, Henan, China
Xin-Ying Ji 11
(10190096@vip.henu.edu.cn) Kaifeng Key Laboratory of Infection and Biological Safety, Henan University College of Medicine,
Kaifeng, Henan, China
Citation: Khan NH, Shahid M,
Wang W, et al. 2022, Structural,
functional, phylogenetic, and
molecular dynamic simulation study
of PEST-containing nuclear protein: Abstract
An e-science view. Gene Protein
Dis, 1(1):65. PEST-containing nuclear protein (PCNP) is a short-lived novel nuclear protein. It has
https://doi.org/10.36922/gpd.v1i1.65
been well evaluated that PCNP mediates the progression of several cancers, but
Received: April 12, 2022 the exact mechanisms are still under investigation. In this study, we provided an
Accepted: May 19, 2022
Published Online: June 3, 2022 e-science view of PCNP protein from the aspects of protein structure, interactions, and
bioinformatics-based analysis related to evolutionary features as well as proteomic
Copyright: © 2022 Author(s).
This is an Open Access article profile. The phylogenetic relationship results reveal that PCNP is closely related to
distributed under the terms of the Pan troglodytes and the Bovidae family, while being distantly related to the Muridae
Creative Commons Attribution family. The analysis of the physicochemical properties of PCNP demonstrated that it
License, permitting distribution,
and reproduction in any medium, is a thermolabile protein which is slightly acidic and hydrophilic in nature. Further,
provided the original work is coexpression and protein-protein interaction analyses were carried out, which
properly cited. demonstrated that the PCNP gene was remarkably expressed with MORF4LI and
Publisher’s Note: AccScience RSL24D1 genes and has close interactions with TRAM1, PSMC6, SRP9, PRKRIR, UHRF2,
Publishing remains neutral with and BMI1 proteins. Gene ontology and pathway enrichment analyses showed that
regard to jurisdictional claims in
published maps and institutional PCNP has a high tendency to work in cell cycle regulation. Moreover, among the
affiliations. four 3D structure generating tools, I-TASSER-generated structure had the highest

Volume 1 Issue 1 (2022) 1 https://doi.org/10.36922/gpd.v1i1.65