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Global Translational Medicine GalNAc AGT ASO reduce atherosclerosis

A B C

D

Figure 5. Effects of hepatocyte-specific antisense oligonucleotides of angiotensinogen versus losartan on liver steatosis. (A) Liver weight at termination.
(B) Liver total cholesterol concentration (normalized by liver weight). (C) Liver triglycerides concentration (normalized by liver weight). (D) Hematoxylin
and eosin (H and E) staining and Oil Red O (ORO) staining of liver sections; scale bar = 200 µm. N = 10 per group.
GalNAc AGT ASO: N-acetylgalactosamine-conjugated antisense oligonucleotides targeting angiotensinogen; PBS: Phosphate-buffered saline.

5. Conclusions angiotensinogen in thoracic aortic aneurysms. Adam
E. Mullick is an employee of Ionis Pharmaceuticals, who
GalNAc AGT ASO not only reduces BP and atherosclerosis, provided the mouse GalNAc AGT ASO. IONIS-AGT-Lrx
but also improves diet-induced liver steatosis in and ION904 are both AGT ASO Ionis programs in clinical
mice. Future studies should investigate whether AGT development. Ionis Pharmaceuticals did not provide any
suppression induces similar effects in humans and non- funding for this study. The other authors report no conflicts.
human primates.
Author contributions
Acknowledgments
Conceptualization: Congqing Wu, Alan Daugherty, Hong
None.
S. Lu
Funding Data curation: Dien Ye, Congqing Wu, Lei Cai, Deborah A.
Howatt, Ching-Ling Liang, Adam E. Mullick
This research work was supported by the National Heart, Investigation: Dien Ye, Congqing Wu, Lei Cai, Deborah A.
Lung, and Blood Institute of the National Institutes of Howatt, Ching-Ling Liang, Ryan E. Temel, Hong S. Lu
Health (R01HL139748 and R00HL145117). The content Supervision: Alan Daugherty, Hong S. Lu
in this article is solely the responsibility of the authors Validation: Dien Ye, Congqing Wu, Yuriko Katsumata,
and does not necessarily represent the official views of the Hong S. Lu
National Institutes of Health. Visualization: Dien Ye, Hong S. Lu
Writing – original draft: Dien Ye, A.H. Jan Danser, Hong
Conflict of interest
S. Lu
Alan Daugherty and Hong S. Lu have submitted a patent Writing – review & drafting: Dien Ye, Congqing Wu, Lei
application for use of antisense oligonucleotides targeting Cai, Deborah A. Howatt, Ching-Ling Liang, Yuriko

Volume 2 Issue 1 (2023) 8 https://doi.org/10.36922/gtm.288