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Juliana R. Dias, et. al.

In direct contact assays no statistical significance was pro ducible nanofiber meshes from materials with
observed between control and samples, meaning that distinct characteristics. The system was used to pro-
the structures, when in contact with cells, does not duce nanofibers from two distinct polymers, using
induce any toxicity. Regarding indirect contact assay, two different solvents, demonstrating its versatility
no leachables delivered from the samples to the medium of the new reassembled apparatus. The fabrication of
presented toxicity. gelatin meshes is particularly relevant; as like other
natural polymers, it is a material very sensitive to the
4. Conclusions environmental conditions, in particular the relative
This paper introduces a solution electrospinning sys- humidity. Keeping the processing parameters stable is
tem developed to produce electrospun meshes for key to obtain high quality and reproducible meshes,
applications in tissue engineering and more specifically i.e., with no beads, resulting in filaments with constant
for wound dressing applications. For the fabrication of diameter and in meshes with high porosity between
the electrospinning system non-conductive materials pores. Meshes did not show any presence of remaining
(cork and polymers) were used to replace metallic solvents, which can be correlated to the lack of toxicity
ones, allowing to obtain a feasible and versatile detected through the biological assays.
laboratory-scale apparatus with ability to produce re- The two selected materials are particularly relevant

Figure 5. Characterization of PCL and gelatin electrospun meshes selected for further analysis. (A) Fiber morphology with 1000×
and 5000× magnifications of PCL meshes; (B) Fiber morphology with 1000× and 5000× magnifications of gelatin meshes crosslinked
with BDDGE; (C) Comparison between PCL and gelatin average fiber diameter; (D) FTIR spectra of PCL and crosslinked gelatin and
(E) Influence of the purpose electrospinning system on fiber deposition over the collector in comparison with initial one comprising a
significant metallic components.

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