Page 40 - IJB-3-2
P. 40
A new design of an electrospinning apparatus for tissue engineering applications
spinning design and nanofibre assemblies. Nano technology,
vol.17(14): 89–106.
https://dx.doi.org/10.1088/0957-4484/17/14/R01
3. Ramakrishna S, Fujihara K, Teo W-E, et al., 2005, An
Introduction to Electrospinning and Nanofibers, World
Scientific, Singapore.
4. Rim N G, Shin C S and Shin H, 2013, Current approaches
to electrospun nanofibers for tissue engineering. Biomedical
Materials, vol.8(1): 014102.
5. Bhardwaj N and Kundu S C, 2010, Electrospinning: A fas-
cinating fiber fabrication technique. Biotechnology Advances,
vol.28(3): 325–347.
Figure 6. Cytotoxicity assay of PCL and gelatin electrospun
meshes in direct contact (DC) and indirect contact (IC) with https://dx.doi.org/10.1016/j.biotechadv.2010.01.004
fibroblasts (hDNF cells), using as control cells seeded on the well. 6. Niu H and Lin T, 2012, Fiber generators in needleless
Statistical significance for p ≤ 0.05 (*).
electrospinning. Journal of Nanomaterials, vol.12: 1–13.
for skin applications. PCL presents high mechanical https//dx.doi.org/10.1155/2012/725950.
properties but, due to its hydrophobic nature, presents 7. Sill T J and von Recum H A, 2008, Electrospinning:
low interaction with cells. Contrary, gelatin displays Applications in drug delivery and tissue engineering.
many integrin-binding sites for cell adhesion, migration, Biomaterials, vol.29(13): 1989–2006.
proliferation, and differentiation due to the abundant https://dx.doi.org/10.1016/j.biomaterials.2008.01.011
Arg–Gly–Asp (RGD) amino acid sequences in its protein 8. Shin S H, Purevdori O, Castano O, et al., 2012, A short
chain, which has been claimed to favor cell behavior.
The combination of both materials may allow to produce review: recent advances in electrospinning for bone tissue
meshes with improved properties. regeneration. Journal of Tissue Engineering, vol.3(1): 1–11.
https://dx.doi.org/10.1177/2041731412443530
Conflict of Interest and Funding
9. Nukavarapu S P, Kumbar S G, Merrell J G, et al., 2014,
No conflict of interest was reported by the authors. Electrospun polymeric nanofibre scaffolds for tissue rege-
This study was supported by the Project PTDC/BBB- neration. In: Laurencin C T and Nair L S (eds), Nano
ECT/2145/2014 and UID/Multi/04044/2013, financed by technology and Tissue Engineering: The Scaffold, 2nd edition,
European Fonds Européen de Développement Régional
(FEDER) through the Portuguese national programs Taylor & Francis, London, 229–254.
Programa Operacional Factores de Competitividade 10. Li W J and Xia Y, 2004, Electrospinning of nanofibres:
(COMPETE), Portugal2020 and Norte2020, and by Reinventing the wheel? Advanced Materials, vol.16(14):
Portuguese funds through Fundação para a Ciência e 1151–1170.
a Tecnologia (FCT). This work is also supported by https://dx.doi.org/10.1002/adma.200400719
a research grant (SFRH/BD/91104/2012) awarded to 11. IME Technologies, n.d., IME Technologies: Your partner in
Juliana Dias by FCT.
electrospinning, viewed January 5, 2017,
Acknowledgements <https://www.imetechnologies.com>
The authors thank CEMUP, University of Porto for the 12. Inovenso, n.d. Inovenso. viewed January 8, 2017,
SEM images. <http://inovenso.com/about/>
References 13. Elmarco, n.d., NanoSpiderTM Electrospinning Technology,
viewed January 16, 2017,
1. Bártolo P, Kruth J-P, Silva J, et al., 2012, Biomedical <http://www.elmarco.com/electrospinning/electrospinning-
production of implants by additive electro-chemical technology/>
and physical processes. CIRP Annals — Manufacturing 14. Har-el Y-E, Gerstenhaber J A, Brodsky R, et al., 2014,
Technology, vol.61(2): 635–655. Electrospun soy protein scaffolds as wound dressings:
https://dx.doi.org/10.1016/j.cirp.2012.05.005 Enhanced reepithelialization in a porcine model of wound
2. Teo W E and Ramakkrishna S, 2006, A review on electro- healing. Wound Medicine, vol.5: 9–15.
128 International Journal of Bioprinting (2017)–Volume 3, Issue 2

