Page 139 - IJB-10-3

International Journal of Bioprinting New challenges in liver tissue engineering

Table 3. Liver cell transplantation for the treatment of liver disease
Biomaterial Cell type Animal model Highlights Refs
Inborn errors of metabolism
Long Evans – Decrease in blood copper.
HA scaffold Fetal rat liver cells Cinnamon rat – Significant reduction in total and direct bilirubin levels. 153
(Wilson’s disease) – Increased albumin production.
Collagen-coated Rat hepatocytes Gunn rat (Crigler– – Maintained decrease in serum total levels of bilirubin. 167
microcarriers Najjar disease) – Presence and persistence of conjugated bilirubin after 3–4 weeks.
Acute liver failure
– Improved survival.
Decellularized rat Human EpCAM+ Wistar rats + ALF – Improvements in ammonia, bilirubin, and albumin levels in 155
liver scaffolds cells (D-GalN) transplanted animals.
– Reduction of prothrombin time in transplanted animals.
HLC-derived – 3D microenvironment supported hepatic differentiation of MSCs.
Decellularized rat mouse NOD-SCID mice + – Increased survival (63%) in transplanted animals. 168
liver scaffolds mesenchymal ALF (CCl ) – Liver functions (albumin, transaminase, and bilirubin levels)
4
stem cells improved after transplantation.
– Reduction of transaminases levels and total bilirubin in transplanted
animals.
PCL-PLA-PAAm- Rat bone marrow Sprague–Dawley – Histology of other relevant organs showed no toxicity associated to
IGF-1/TNF-α mesenchymal rats With ALF the scaffold. 159
scaffolds stem cells (D-GalN/LPS)
– In vitro studies showed the differentiation potential of MSCs
cultured in the scaffolds.
Nude mice +
Col I and chitosan L02 cells ALF (radiation + – Increased survival of transplanted animals after 14 days. 162
printed scaffold – Decreased levels of ALT and AST.
hepatectomy)
– Increased survival in transplanted animals.
– Significant reduction of transaminases levels in animals
Primary human NOD/SCID with transplanted with PHH-laden scaffolds compared to ALF animals
Gel-HA scaffold 90
hepatocytes ALF (APAP) and ALF animals only treated with cells.
– Reduction of oxidative stress and proinflammatory cytokines in
animals transplanted with PHH-scaffolds.
– Increased survival (37%) in transplanted animals.
Pullulan and HepaRG NOD/SCID mice – Human albumin remained stable in sera for 28 days. 117
dextran scaffolds with ALF (APAP)
– Successful engraftment and survival of transplanted cells.
– Increased survival in transplanted animals.
– Reduced levels of transaminases and bilirubin levels in animals
transplanted with microbeads.
Rat primary Sprague–Dawley rat
Alg microbeads – No sign of inflammation or adhesion was observed in treated 150
hepatocytes + ALF (D-GalN)
animals.
– Complete normal lobular architecture in transplanted animals after
1 week.
Carboxymethyl- BALB/c mice + – Increased survival of transplanted animals; survival was higher in
hexanoyl chitosan iPSC-HLC ALF (thioacetamide functionalized hydrogels containing HGF. 152
HGF-releasing overdose) – HLC delivered in hydrogels releasing HGF showed higher
hydrogels antioxidant and antiapoptotic activities that reduced necrosis.
Mebiol TGP Wistar rat + ALF – Increased survival of transplanted animals.
(PNIPAAm-PEG) Rat hepatocytes (D-GalN) – Reduction of transaminases and bilirubin levels after 151
hydrogels transplantation.
– Higher survival of animals transplanted with cell-sheet HLC than
Rag2/Il2rg double-
Human iPSC- knockout mice + those transplanted intrasplenically with HLC.
Cell sheets – Increased albumin levels in transplanted animals. 165
HLC partial hepatectomy – Reduced transaminase levels in animals transplanted with cell-sheet
(70%) + ALF (CCl )
4 HLC.
(Continued...)

Volume 10 Issue 3 (2024) 131 doi: 10.36922/ijb.2706