Page 512 - IJB-10-5

International Journal of Bioprinting 3D model of neurogenesis in Alzheimer’s disease

Table 1. Reagents and equipment.
Reagent Or Resource Source Identifier

Reagents, antibodies, supplements, and other chemicals

Accutase Gibco, USA A1110501
Alexa Fluor 488 Goat anti-Rabbit Invitrogen, USA A11008

Alexa Fluor 647 Goat anti-Chicken Invitrogen, USA A21449

Aβ-protein (Human, Aβ ) Peptide Institute, INC, Japan 4349-v
1-42
B-27 Supplement Minus Vitamin A Gibco, USA 12587010

Calcium Chloride (CaCl ) Synth, Brazil C201301AH
2
CellROX (Green Reagent) Invitrogen, USA C10444

DAPI Sigma-Aldrich, USA 62248
Dimethyl sulfoxide (DMSO) Sigma-Aldrich, USA 1003246030

Dulbecco’s modified eagle medium/Nutrient Gibco, USA 12400024
mixture F-12 (DMEM/F12)

Fetal Bovine Serum (FBS) Gibco, USA 12657-029

Gelatin from porcine skin (GEL) Sigma-Aldrich, USA G2500, 9000-70-8
Bovine sodium heparin Kin Master, Brazil 50.1000.01

Human FGF-basic (FGF-2/bFGF) Gibco, USA PHG0026
L-glutamine MP Biomedicals, USA 101806

LIVE/DEAD assay (Cell Imaging Kit 488/570) Invitrogen, USA R37601

Penicillin/streptomycin Gibco, USA 15140-122
Phalloidin Sigma-Aldrich, USA 49409

poly (2-hydroxyethylmethacrylate) (poly-hema) Sigma-Aldrich, USA P3932
Primary MAP-2 Antibody (Rb X MAP2) Sigma-Aldrich, USA ab5622

Primary Nestin Antibody (Chk pAb to Nestin) Abcam, USA ab134017

Recombinant Human EGF Protein R&D System, USA 236-EG-01M
Resazurin powder Sigma-Aldrich, USA R7017

Sodium alginate (ALG, Alginic acid sodium salt Sigma-Aldrich, Norway A1112
from brown algae)

Triton X-100 Sigma-Aldrich, USA 9002-93-1
Trypsin Gibco, USA 15090046

Volume 10 Issue 5 (2024) 504 doi: 10.36922/ijb.3751