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REVIEW ARTICLE

Collagen as Bioink for Bioprinting: A Comprehensive

Review

Egor Olegovich Osidak *, Vadim Igorevich Kozhukhov , Mariya Sergeevna Osidak ,
1,2
2,3
1
Sergey Petrovich Domogatsky 2,3
rd
1 Imtek Ltd., 3 Cherepkovskaya 15A, Moscow, Russia
2 Gamaleya Research Institute of Epidemiology and Microbiology Federal State Budgetary Institution, Ministry of Health of
the Russian Federation, Gamalei 18, Moscow, Russia
3 Russian Cardiology Research and Production Center Federal State Budgetary Institution, Ministry of Health of the Russian
Federation, 3 Cherepkovskaya 15A, Moscow, Russia

Abstract: Biomaterials made using collagen are successfully used as a three-dimensional (3D) substrate for cell culture and
considered to be promising scaffolds for creating artificial tissues. An important task that arises for engineering such materials
is the simulation of physical and morphological properties of tissues, which must be restored or replaced. Modern additive
technologies, including 3D bioprinting, can be applied to successfully solve this task. This review provides the latest evidence
on advances of 3D bioprinting with collagen in the field of tissue engineering. It contains modern approaches for printing pure
collagen bioinks consisting only of collagen and cells, as well as the obtained results from the use of pure collagen bioinks in
different fields of tissue engineering.

Keywords: Collagen, Three-dimensional bioprinting, Tissue engineering, Cell-laden hydrogels
*Corresponding Author: Egor Olegovich Osidak, Gamaleya Research Institute of Epidemiology and Microbiology Federal State Budgetary
Institution, Ministry of Health of the Russian Federation, Gamalei 18, Moscow, Russia; OsidakEgor_egorosidak@gmail.com
Received: March 02, 2020; Accepted: March 16, 2020; Published Online: April 21, 2020

(This article belongs to the Special Section: Bioprinting in Russia)

Citation: Osidak EO, Kozhukhov VI, Osidak MS, et al., 2020, Collagen as Bioink for Bioprinting: A Comprehensive Review.
Int J Bioprint, 6(3): 270. DOI: 10.18063/ijb.v6i3.270.

1 Introduction patient into account at the same time . Moreover,
[2]
the use of this technology allows building complex
For the past few years, additive technologies, structures that are already colonized with cells at
including the technology of three-dimensional the moment of bioprinting. Cell-laden hydrogels,
(3D) bioprinting, have emerged into a rapidly
developing tissue engineering sphere . These which are also called bioinks, are used to create
[1]
[3]
technologies allow creating layer-by-layer such structures .
assembled structures with a specific pore size and Synthetic polymers, such as poly(ethylene
porosity that promotes the restoration of defects of glycol), as well as native proteins, such as collagen,
soft or hard tissues. Another indisputable advantage can be used as a structural basis for such hydrogels [3,4] .
of the 3D bioprinting is that it allows creating Collagen-containing hydrogels are currently the
personalized implants for the specific needs of most popular cell scaffold and material for tissue
a patient, taking the individual features of the engineering, especially if working with cells is

© 2020 Osidak, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International
License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the
original work is properly cited.
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