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International Journal of Bioprinting Collagen hydrolysate-loaded ODMA/PEGDMA scaffold

of the properties of ODMA resin mixed with PEGDMA Methodology: K. Pasanaphong, A. Suksuwan, R.
at varying concentrations revealed its compatibility with Hemstapat, T. Tawonsawatruk, S. Boonyagul,
DLP 3D printing, except for the lowest concentration N. Tanadchangsaeng
(0.625% w/v), which exhibited reduced formability. SEM Resources: K. Pasanaphong, N. Srikaew, T. Tawonsawatruk,
analysis of the cell scaffolds fabricated from this resin S. Wangtueai, N. Tanadchangsaeng
highlighted differences in surface morphology, with the Software: A. Suksuwan, R. Hemstapat, T. Tawonsawatruk,
0.625% concentration exhibiting a rough and irregular S. Wangtueai, N. Tanadchangsaeng
surface compared to the smoother surfaces obtained at Supervision: A. Suksuwan, R. Hemstapat, T. Tawonsawatruk,
higher concentrations. For the mechanical property test, it S. Boonyagul, N. Tanadchangsaeng
was found that PEGDMA with 10% ODMA, the highest Project administration: N. Tanadchangsaeng
Validation: All authors
compared to 0.625%, 1.25%, 2.5%, and 5.0% concentration
studied here, had the maximum strain and stress values Visualization: K. Pasanaphong, A. Suksuwan,
N. Srikaew
compared to the other concentrations. Cytotoxicity tests on Writing – original draft: K. Pasanaphong, A. Suksuwan, N.
fibroblast cells in media containing CH post-sterilization Srikaew, R. Hemstapat, S. Boonyagul, P. Wongsirichot,
reported promising results; fluorescent staining revealed N. Tanadchangsaeng
a high number of viable fibroblasts in all samples. Writing – review & editing: K. Pasanaphong, A. Suksuwan,
Chondrocyte cell viability on scaffolds displayed varying N. Srikaew, R. Hemstapat, S. Boonyagul, P.
survival rates across different scaffold compositions, Wongsirichot, N. Tanadchangsaeng
with ODMA/PEGDMA and ODMA/PEGDMA/CH
composites exhibiting significantly higher viability Conflict of interest
compared to PEGDMA alone. These findings demonstrate The authors declare no competing interests.
the potential of these materials for biomedical applications,
particularly in cartilage tissue engineering, where scaffold Ethics approval and consent to participate
biocompatibility and mechanical properties are crucial.
All human samples used in this study were approved by
Acknowledgments the Human Research Ethics Committee of the Faculty
of Medicine Ramathibodi Hospital, Mahidol University
The authors are thankful to Anuchan Panaksri for (COA. MURA2022/469). A written informed consent
proofreading and editing this manuscript. document was signed by the enrolled participant prior
to the initiation of the study. The demographic data
Funding of patients cannot be tracked to their profiles. We
The present study was supported by the National Research confirm that all methods and experimental protocols
Council of Thailand (NRCT) via the Invention to Business were carried out in accordance with relevant guidelines
(I-2B) Program (contract number: N82A670613). This and regulations.
research was also supported by Rangsit University. This Consent for publication
research project was funded by Fundamental Fund 2023,
Chiang Mai University. Not applicable.

Author contributions Availability of data

Conceptualization: K. Pasanaphong, A. Suksuwan, The datasets used and/or analyzed in the study are
R. Hemstapat, T. Tawonsawatruk, S. Boonyagul, available from the corresponding author upon
N. Tanadchangsaeng reasonable request.
Data curation: K. Pasanaphong, N. Srikaew, R. Hemstapat, References
P. Wongsirichot, N. Tanadchangsaeng
Formal analysis: K. Pasanaphong, A. Suksuwan, N. 1. Sophia Fox AJ, Bedi A, Rodeo SA. The basic science of
Srikaew, R. Hemstapat, N. Khamthong, S. Boonyagul, articular cartilage: structure, composition, and function.
P. Wongsirichot, N. Tanadchangsaeng Sports Health. 2009; 1:461-468.
Funding acquisition: S. Wangtueai, N. Tanadchangsaeng doi: 10.1177/1941738109350438
Investigation: K. Pasanaphong, A. Suksuwan, N. Srikaew, 2. Wojcieszek A, Kurowska A, Majda A, Liszka H, Gądek
N. Khamthong, S. Boonyagul, N. Tanadchangsaeng A. The impact of chronic pain, stiffness and difficulties in

Volume 10 Issue 6 (2024) 357 doi: 10.36922/ijb.4385