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Latest Scientific Pathways on Tissue Spheroids: Opportunities to Innovate
Table 4. Global trend: Spheroid formation.
Article Year/Journal Impact Analysis
Miller, A. J. et al. “Generation 2019/Nature “Protocol that recapitulates several stages like induction,
of lung organoids from human Protocols patterning, lung specification, budding, morphogenesis; to
pluripotent stem cells in vitro” . differentiate human pluripotent stem cells (hPSCs) into ventral–
[28]
anterior foregut spheroids and further into two distinct types
of organoids: human lung organoids and bud tip progenitor
organoids.”
Lee, W. et al. ” Dispersible 2019/Nature “Development of ultrasoft mechanosensors that visibly deform
hydrogel force sensors reveal Communications under <10 Pascals of cell-generated stress. By incorporating
patterns of solid mechanical mechanosensors into multicellular spheroids, the patterns
stress in multicellular spheroid of internal stress that arise during spheroid formation where
cultures” captured. This technique can provide a quantitative basis to
[29]
design tissues that leverage the mechanical activity of constituent
cells to evolve towards a desired form and function.” In this
research, HS-5 fibroblasts were used as well as an aqueous two-
phase droplet printing technique by an automated liquid handler
in a concentration of 6×107 cells/mL.
Heo, D. N. et al. ” Synergistic 2019/Acta “To enhance stem cell function and generate pre-vascularized
interplay between human MSCs Biomaterialia network, a collagen/fibrin hydrogel was employed as
and HUVECs in 3D spheroids an encapsulation matrix for the incorporation of human
laden in collagen/fibrin hydrogels mesenchymal stem cell/human umbilical vein endothelial cell
for bone tissue engineering” [30] (MSC/HUVEC) spheroids, and their cellular behavior (including
cell viability, morphology, proliferation, and gene expression
profile) was investigated and compared to that of cell suspension-
or MSC spheroids-laden hydrogels.” In this study, microwells
in AggreWell plates were used. Cell suspension at a density of
1.2×106 cells/well was seeded. MSC-only and MSC/HUVEC
(75%/25%) spheroids were used.

Figure 1. Distribution of scientific publications by categories.

authors were Vladimir Mironov and Vladimir Kasyanov Figure 2. Distribution of the subject areas of publication on the use
with 24 and 14 publications each. The third most prolific of tissue spheroids.
author is Gerhard Björn Stark with 13 scientific articles.
These findings can correlate directly to the recently made by Russian bioprintists that have contributed to
published paper “Bioprinting in the Russian Federation: global technology in the field, such as the development
Can Russians Compete?” by Peter Timashev and Vladimir of original 3D bioprinters, natural bioinks and the world’s
[34]
Mironov in which they state five main achievements first functional and vascularized organ construct .

140 International Journal of Bioprinting (2021)–Volume 7, Issue 1