Page 9 - IJB-9-2

International Journal of Bioprinting

REVIEW ARTICLE
Biomaterial inks for extrusion-based

3D bioprinting: Property, classification,
modification, and selection

Xiaorui Li 1,2† , Fuyin Zheng 1,2† *, Xudong Wang , Xuezheng Geng , Shudong
1
1
Zhao , Hui Liu , Dandan Dou , Yubing Leng , Lizhen Wang *, Yubo Fan *
1
1
1,2
1,2
1,2
1
1 Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical
Engineering, and with the School of Engineering Medicine, Beihang University, Beijing 100083,
China
2 Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing 100083,
China
(This article belongs to the Special Issue: 3D Bioprinting with Photocurable Bioinks)
Abstract
Three-dimensional (3D) extrusion-based bioprinting is the most widely used
bioprinting technology to fabricate bionic tissue or organ constructs by combining
biomaterial ink and living cells for tissue engineering and regenerative medicine.
One critical issue of this technique is the selection of suitable biomaterial ink to
† These authors contributed equally simulate extracellular matrix (ECM) that provides mechanical support for cells and
to this work. regulates their physiological activities. Previous studies have demonstrated that
*Corresponding authors: it is an enormous challenge to form and maintain reproducible 3D constructs and
Fuyin Zheng eventually achieve the balance among biocompatibility, mechanical properties, and
(zhengfuyin@buaa.edu.cn)
Lizhen Wang printability. This review highlights the properties of extrusion-based biomaterial
(lizhenwang@buaa.edu.cn) inks and recent developments as well as details various biomaterial inks classified by
Yubo Fan (yubofan@buaa.edu.cn) their function. Key approaches related to their modification methods according to
Citation: Li X, Zheng F, Wang X, the functional requirements are also discussed, along with the selection strategies
et al., 2023, Biomaterial inks for by varying extrusion paths and methods in extrusion-based bioprinting. This
extrusion-based 3D bioprinting:
Property, classification, modification, systematical review will assist researchers in identifying the most suitable extrusion-
and selection. Int J Bioprint, 9(2): based biomaterial inks based on their requirements, as well as in elaborating current
649. challenges and prospects of extrudable biomaterial inks in the field of bioprinting of
https://doi.org/10.18063/ijb.v9i2.649 in vitro tissue models.
Received: February 24, 2022
Accepted: September 07, 2022
Published Online: December 9, Keywords: 3D bioprinting; Extrusion-based method; Bioink; Biomaterials; Tissue
2022 engineering
Copyright: © 2022 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution 1. Introduction
and reproduction in any medium,
provided the original work is Three-dimensional (3D) bioprinting technology has been widely used to construct
properly cited. in vitro bionic functional tissues and organs with complex microarchitecture
Publisher’s Note: Whioce and physiological function for tissue repair and regeneration. This technique can
Publishing remains neutral with automatically and accurately control the 3D microstructure and cell distribution as well
regard to jurisdictional claims in as locate biological signals within scaffolds in tissue engineering. The application of
published maps and institutional
affiliations. engineered 3D vascularized tissue constructs derived from bioprinting can be further

Volume 9 Issue 2 (2023) 1 https://doi.org/10.18063/ijb.v9i2.649