Page 316 - IJB-9-3

International Journal of Bioprinting Bioprinted stem cell niche regenerates muscles

3D construct can be released slowly with time into adjacent References
tissues, which could be beneficial for stem cell survival and
persistence surrounding the implanted construct. 1. Sacco A, Mourkioti F, Tran R, et al., 2010, Short telomeres
and stem cell exhaustion model Duchenne muscular
5. Conclusion dystrophy in mdx/mTR mice. Cell, 143(7):1059–1071.

Our current result indicates that the reactivation of Notch https://doi.org/10.1016/j.cell.2010.11.039
signaling in dystrophic muscle is effective in promoting 2. Jang YC, Sinha M, Cerletti M, et al., 2011, Skeletal muscle
self-renewal of muscle stem cells, and demonstrates that stem cells: Effects of aging and metabolism on muscle
bioprinting of Notch activator can be applied to serve as regenerative function. Cold Spring Harb Symp Quant Biol,
stem cell niche and improve the efficacy of muscle stem 76:101–111.
cell transplantation in diseased muscle. We believe that https://doi.org/10.1101/sqb.2011.76.010652
the same technology could possibly be adapted for the
treatment of a wide variety of traumatic and atraumatic 3. Usas A, Maciulaitis J, Maciulaitis R, et al., 2011, Skeletal
muscle conditions in the future. muscle-derived stem cells: Implications for cell-mediated
therapies. Medicina (Kaunas), 47(9):469–479.
Acknowledgments 4. Ikezawa M, Cao B, Qu Z, et al., 2003, Dystrophin delivery in
dystrophin-deficient DMDmdx skeletal muscle by isogenic
We greatly appreciate the fundings or supports from muscle-derived stem cell transplantation. Hum Gene Ther,
Shandong First medical University and Shandong 14(16):1535–1546.
Academy of Medical Science, and Steadman Philippon
Research Institute (SPRI). https://doi.org/10.1089/104303403322495043
5. Winkler T, von Roth P, Matziolis G, et al., 2009, Dose-response
Conflict of interest relationship of mesenchymal stem cell transplantation and
The authors declare no conflict of interest. functional regeneration after severe skeletal muscle injury in
rats. Tissue Eng Part A, 15(3):487–492.
Author contributions https://doi.org/10.1089/ten.tea.2007.0426
Conceptualization: Lee Weiss, Phil Campbell, Johnny 6. Tremblay JP, Malouin F, Roy R, et al., 1993, Results of a
Huard, Xiaodong Mu triple blind clinical study of myoblast transplantations
Formal analysis: Zewei Sun, Xianlin Yue, Lei Liu, Ying Li without immunosuppressive treatment in young boys
Investigation: Zewei Sun, Xianlin Yue, Lei Liu, Ying Li, with Duchenne muscular dystrophy. Cell Transplant, 2(2):
Jie Cui 99–112.
Methodology: Zewei Sun, Xianlin Yue, Lee Weiss, Phil 7. Drowley L, Okada M, Beckman S, et al., 2010, Cellular
Campbell, Johnny Huard, Yanling Mu, Xiaodong Mu antioxidant levels influence muscle stem cell therapy. Mol
Visualization: Zewei Sun, Xianlin Yue, Johnny Huard, Ther, 18(10):1865–1873.
Xiaodong Mu https://doi.org/10.1038/mt.2010.160
Writing – original draft: Zewei Sun, Xianlin Yue, Johnny
Huard, Xiaodong Mu 8. Maclean S, Khan WS, Malik AA, et al., 2012, The potential
Writing – review & editing: Zewei Sun, Xianlin Yue, Johnny of stem cells in the treatment of skeletal muscle injury and
disease. Stem Cells Int, 2012:282348.
Huard , Yanling Mu, Xiaodong Mu
https://doi.org/10.1155/2012/282348
Ethics approval and consent to participate 9. Gussoni E, Blau HM, Kunkel LM, 1997, The fate of

All procedures were approved by the Institutional Animal individual myoblasts after transplantation into muscles of
Care and Use Committee (IACUC) at the University of DMD patients. Nat Med, 3(9):970–977.
Pittsburgh (IACUC-1109718). 10. Karpati G, Holland P, Worton RG, 1992, Myoblast transfer
in DMD: Problems in the interpretation of efficiency. Muscle
Consent for publication Nerve, 15(10):1209–1210.

Not applicable. https://doi.org/10.1002/mus.880151016
11. Mayeuf A, Relaix F, 2011, Notch pathway: From
Availability of data development to regeneration of skeletal muscle. Med Sci
The data that support the findings of this study are available (Paris), 27(5):521–526.
from the corresponding author upon reasonable request. https://doi.org/10.1051/medsci/2011275018

Volume 9 Issue 3 (2023) 308 https://doi.org/10.18063/ijb.711