Page 172 - IJB-9-4

International Journal of Bioprinting

RESEARCH ARTICLE
Multi-scale vascularization strategy for

3D-bioprinted tissue using coaxial core–shell
pre-set extrusion bioprinting and biochemical

factors

Jae-Hun Kim , Minji Park , Jin-Hyung Shim , Won-Soo Yun , Songwan Jin *
1
2
2,3
2,3
2,3
1 Department of Mechanical System Engineering, Graduate School of Knowledge-based Technology
and Energy, Tech University of Korea, Siheung-si, Gyeonggi-do, Republic of Korea
2 T&R Biofab, Siheung-si, Gyeonggi-do, Republic of Korea
3 Department of Mechanical Engineering, Tech University of Korea, Siheung-si, Gyeonggi-do,
Republic of Korea

Abstract

Three-dimensional bioprinting is a key technology in bioartificial organ production.
However, production of bioartificial organs has significant limitations because it is
hard to build vascular structures, especially capillaries, in printed tissue owing to
its low resolution. As the vascular structure plays a critical role in delivering oxygen
and nutrients to cells and removing metabolic waste, building vascular channels
in bioprinted tissue is essential for bioartificial organ production. In this study, we
demonstrated an advanced strategy for fabricating multi-scale vascularized tissue
using a pre-set extrusion bioprinting technique and endothelial sprouting. Using a
*Corresponding author:
Songwan Jin coaxial precursor cartridge, mid-scale vasculature-embedded tissue was successfully
(songwan@tukorea.ac.kr) fabricated. Furthermore, upon generating a biochemical gradient environment
Citation: Kim J-H, Park M, in the bioprinted tissue, capillaries were formed in this tissue. In conclusion, this
Shim J-H, et al., 2023, Multi- strategy for multi-scale vascularization in bioprinted tissue is a promising technology
scale vascularization strategy for for bioartificial organ production.
3D-bioprinted tissue using coaxial
core–shell pre-set extrusion
bioprinting and biochemical factors.
Int J Bioprint, 9(4): 726. Keywords: 3D bioprinting; Biochemical gradient; Endothelial sprouting; Pre-set
https://doi.org/10.18063/ijb.726 extrusion; Vascularized tissue
Received: December 21, 2022
Accepted: March 01, 2023
Published Online: April 4, 2023
Copyright: © 2023 Author(s). 1. Introduction
This is an Open Access article
distributed under the terms of the The organ shortage crisis is a long-standing and deepening problem in organ
Creative Commons Attribution transplantation . The most promising technology for addressing organ shortage is the
[1]
License, permitting distribution,
and reproduction in any medium, production of bioartificial organs as a substitute for damaged or dysfunctional organs.
[2]
provided the original work is However, methods to produce bioartificial organ such as genetically modified xenograft ,
properly cited. cell-laden biomaterial molding , and recellularization of decellularized organ have
[3]
[4]
Publisher’s Note: Whioce problems such as latent immunoreaction, low reproducibility, and low resolution
Publishing remains neutral with to precise positioning of multiple types of cells. Three-dimensional (3D) bioprinting
regard to jurisdictional claims in
published maps and institutional is a promising technology in the field of bioartificial organ manufacturing, owing to
affiliations. the possibility of 3D patterning on a pre-designed position with a software-controlled

Volume 9 Issue 4 (2023) 164 https://doi.org/10.18063/ijb.726