Page 9 - IJB-9-4

International Journal of Bioprinting

RESEARCH ARTICLE
Biomimetic three-dimensional glioma model

printed in vitro for the studies of glioma cells and
neurons interactions

Luge Bai , Zhiyan Hao , Sen Wang , Jiajia Zhou , Siqi Yao , Na Pei ,
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Hui Zhu , Kun Zhang , Rui L. Reis , J. Miguel Oliveira , Jiankang He ,
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Dichen Li , Xinggang Mao *, Ling Wang *
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1 School of Mechanical Engineering, Xi’an Jiaotong University, Xi’an 710054, Shaanxi, China
2 NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices,
Xi’an Jiaotong University, Xi’an 710054, Shaanxi, China
3 Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an,
Shaanxi, China
4 3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics,
University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering
and Regenerative Medicine, AvePark, Zona Industrial da Gandra, 4805-017 Barco -Guimarães,
Portugal
5
ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, Portugal
6 Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University, Xi’an, Shaanxi,
China
(This article belongs to the Special Issue: Advances in 3D bioprinting based on lab-on-a-chip platforms for
precision oncology)
*Corresponding authors:
Ling Wang Abstract
(menlwang@xjtu.edu.cn)
Xinggang Mao The interactions between glioma cells and neurons are important for glioma
(xgmao@fmmu.edu.cn) progression but are rarely mimicked and recapitulated in in vitro three-dimensional
Citation: Bai L, Hao Z, Wang S, (3D) models, which may affect the success rate of relevant drug research and
et al., 2023, Biomimetic three- development. In this study, an in vitro bioprinted 3D glioma model consisting of
dimensional glioma model printed an outer hemispherical shell with neurons and an inner hemisphere with glioma
in vitro for the studies of glioma
cells and neurons interactions. cells is proposed to simulate the natural glioma. This model was produced by
Int J Bioprint, 9(4): 715. extrusion-based 3D bioprinting technology. The cells survival rate, morphology, and
https://doi.org/10.18063/ijb.715 intercellular Ca concentration studies were carried out up to 5 days of culturing.
2+
Received: November 25, 2022 It was found that neurons could promote the proliferation of glioma cells around
Accepted: January 10, 2023 them, associate the morphological changes of glioma cells to be neuron-like, and
Published Online: March 21, 2023
increase the expression of intracellular Ca of glioma cells. Conversely, the presence
2+
Copyright: © 2023 Author(s). of glioma cells could maintain the neuronal survival rate and promote the neurite
This is an Open Access article outgrowth. The results indicated that glioma cells and neurons facilitated each other
distributed under the terms of the
Creative Commons Attribution implying a symbiotic pattern established between two types of cells during the early
License, permitting distribution stage of glioma development, which were seldom found in the present artificial
and reproduction in any medium, glioma models. The proposed bioprinted glioma model can mimic the natural
provided the original work is
properly cited. microenvironment of glioma tissue, provide an in-depth understanding of cell–cell
interactions, and enable pathological and pharmacological studies of glioma.
Publisher’s Note: Whioce
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: In vitro 3D model; Glioma model; 3D bioprinting; Glioma cells; Neurons
affiliations.

Volume 9 Issue 4 (2023) 1 https://doi.org/10.18063/ijb.715