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International Journal of Bioprinting Laser transfer for CTC isolation

Figure 8. Isolated CTC proliferation assay. MDA-MB-231 were cultured in a 96-well plate with RPMI culture media enriched with FBS (10%) and
[78]
acclimated media (10%) . One microliter of control cells was taken from the sample prior to the laser isolation and cultured in the same conditions.
Dimension bar represents 100 µm.

Figure 9. Complete enrichment-identification-isolation workflow. The proposed approach follows a standard enrichment-identification-isolation process
but due to the negative selection and laser transfer using a BA-LIFT approach, the CTCs remain completely unaltered.

Acknowledgments Incyte, Servier, Lilly, Roche, and Daiichi Sankyo; is in
the Speakers’ Bureau of Rovi, Stada, Menarini, Bayer,
None.
Merck Serono, Amgen, Lilly, Astra Zeneca, Sanofi, BMS,
Funding Pfizer, and Daiichi Sankyo; and has been sponsored
by Roche, Amgen, Merck Serono, Celgene, and Astra
This work was partially supported by Comunidad de Zeneca to cover expenses in travel and accommodations.
Madrid Government/Fondo Social Europeo , S2018/BAA- Sara Lopez-Tarruella has a consulting or advisory role
4480, Biopieltec-CM, and Spanish Ministry for Education at AstraZeneca, Novartis, Roche, Pfizer, Pierre Fabre,
FPU Grant 2015/05605. Lilly, Seagen, Daiichi Sankyo, Gilead Sciences, MSD,
GlaxoSmithKline and Veracity; and is in the Speakers’
Conflict of interest Bureau of Lilly. Miguel Martin has a consulting or
Andres Muñoz has a consulting or advisory role at Sanofi, advisory role at Roche/Genentech, Novartis, Pfizer, Lilly,
Celgene, Pfizer, BMS, Leo Pharma, Astra Zeneca, MSD, AstraZeneca, Taiho Pharmaceutical, and PharmaMar; is in

Volume 9 Issue 4 (2023) 85 https://doi.org/10.18063/ijb.720