Page 138 - ITPS-7-2

INNOSC Theranostics

and Pharmacological Sciences

ORIGINAL RESEARCH ARTICLE
The effect of dapsone on skin flap survival

depends on modulation of inflammatory
response and VEGF expression

1,4
Abolfazl Badripour 1,2,3 , Anahita Najafi , Zahra Ebrahim Soltani ,
1,2
Alireza Hasanzadeh , Mohamad Behzadi , Alireza Rahbar ,
1,4
5
1,4
Armaghan Ahangarishizary , Seyed Mohsen Ahmadi-Tafti ,
3,6
1,4
Mohammad Ashouri and Ahmadreza Dehpour *
3,6
1,7
1 Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
2 Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical
Sciences, Tehran, Iran
3 Colorectal Surgery Research Center, Imam Khomeini Hospital Complex, Tehran University of
Medical Sciences, Tehran, Iran
4 Department of Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Department of Surgery, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran
6 Department of Surgery, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences,
Tehran, Iran
7 Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran,
Iran
Abstract

The random-pattern skin flap is a common method used for reconstructing skin
*Corresponding author: defects. However, flap ischemia necrosis remains a significant challenge in plastic
Ahmadreza Dehpour surgery. Strategies aimed at reducing persistent inflammation and promoting blood
(dehpoura@sina.tums.ac.ir) supply through angiogenesis have been identified as crucial for improving flap survival.
Citation: Badripour A, Najafi A, Dapsone, a chemotherapeutic agent known for its anti-inflammatory properties through
Soltani ZE, et al. The effect of multiple pathways, is of interest in this regard. This study aims to investigate the effect of
dapsone on skin flap survival
depends on modulation of dapsone on random-pattern flap survival in rats, along with its impact on inflammation
inflammatory response and VEGF and angiogenesis. The ischemia/reperfusion (I/R) injury rat models were created using
expression. INNOSC Theranostics a caudal-based dorsal skin flap with delayed I/R. Twenty-four male Sprague Dawley rats
and Pharmacological Sciences.
2024;7(2):2241. were divided into control, sham, and two treatment groups receiving dapsone at doses of
th
doi: 10.36922/itps.2241 12.5 mg/kg/day and 5 mg/kg/day, respectively. On the 7 post-operative day, flap survival
was evaluated. Neutrophil infiltration and ulceration were measured through microscopic
Received: November 13, 2023
examination, and interleukin (IL)-8 levels through enzyme-linked immunosorbent assay.
Accepted: January 25, 2024 Expression levels of vascular endothelial growth factor (VEGF) and tumor necrosis
Published Online: April 16, 2024 factor-alpha (TNF-α) were determined using an immunohistochemistry (IHC) array. The
findings revealed an increased flap survival on day 7 post-operation following systemic
Copyright: © 2024 Author(s).
This is an Open-Access article administration of dapsone for 5 consecutive days. Dapsone at both dosages significantly
distributed under the terms of the reduced the ulcer thickness, neutrophil infiltration, and IL-8 levels. The IHC results revealed
Creative Commons Attribution that VEGF expression was significantly higher in the treatment groups compared to the
License, permitting distribution,
and reproduction in any medium, control group. Moreover, TNF-α expression was significantly lower in the treatment
provided the original work is groups compared to the control group. In conclusion, we confirmed that treatment with
properly cited.
dapsone promotes skin flap survival, and this effect aligned with a reduction in persistent
Publisher’s Note: AccScience inflammation and the enhancement of VEGF. Nonetheless, more studies are required to
Publishing remains neutral with elucidate the precise anti-inflammatory mechanism of dapsone in I/R injuries.
regard to jurisdictional claims in
published maps and institutional
affiliations. Keywords: Dapsone; Inflammation; Ischemia/reperfusion injury; Skin flap; VEGF

Volume 7 Issue 2 (2024) 1 doi: 10.36922/itps.2241