Page 40 - JCTR-11-1

Journal of Clinical and

Translational Research

ORIGINAL ARTICLE
Hesperidin enhances the repair and

regeneration of full-thickness dermal wounds in
mice exposed to fractionated γ-radiation

Ganesh Chandra Jagetia* and Kota V. N. M. Rao

Department of Zoology, School of Life Sciences, Mizoram University, Aizawl, Mizoram, India
(This article belongs to the Special Issue: Innovations in wound healing)

Abstract

Background and aim: Neoplasms are often clinically treated with radiation
therapy, which is known for its therapeutic benefits. However, pre-surgical
radiotherapy is associated with a high incidence of surgical wound healing
complications, necessitating the exploration of new pharmacological approaches.
This study investigates the effects of 100 mg/kg oral hesperidin on mice undergoing
*Corresponding author: hemi-body irradiation with varying fractionated γ-radiation doses. Methods: Mice
Ganesh Chandra Jagetia
(gc.jagetia@gmail.com) were exposed to 10, 20, or 40 Gy doses of fractionated γ-radiation (2 Gy/day)
below the rib cage. Full-thickness excision wounds were created on their dorsum,
Citation: Jagetia GC, Rao KVNM.
Hesperidin enhances the repair and wound contractions were assessed periodically through video imaging.
and regeneration of full-thickness Biochemical profiles were analyzed by measuring collagen content, DNA synthesis,
dermal wounds in mice exposed and nitric oxide (NO) levels in granulation tissue, as well as glutathione (GSH)
to fractionated γ-radiation. J Clin
Transl Res. 2025;11(1):34-55. levels, GSH peroxidase (GSHpx) activity, and lipid peroxidation (LPx) in the skin.
doi: 10.36922/jctr.24.00049 Histological examinations were conducted at various post-irradiation intervals
Received: July 31, 2024 to assess skin regeneration. Results: Fractionated irradiation delayed wound
contraction and prolonged mean wound healing time (MHT) in a dose-dependent
1st revised: November 13, 2024
manner, reducing collagen content, DNA synthesis, and NO levels in granulation
2nd revised: November 20, 2024 tissue. In addition, irradiation decreased GSH concentrations and GSHpx activity
Accepted: January 17, 2025 while increasing LPx, with severity correlating to radiation dose. Fibroblast and
vascular densities also declined following fractionated irradiation. In contrast,
Published online: February 7,
2025 pre-treatment with hesperidin significantly enhanced wound contraction,
reduced MHT, and increased collagen levels, DNA synthesis, and NO production.
Copyright: © 2025 Author(s).
This is an open-access article Furthermore, hesperidin treatment elevated GSH levels and GSHpx activity while
distributed under the terms of the decreasing radiation-induced LPx. In addition, hesperidin pre-treatment improved
Creative Commons AttributionNon- collagen deposition and fibroblast and vascular densities. Conclusion: This study
Commercial 4.0 International (CC
BY-NC 4.0), which permits all demonstrates that hesperidin accelerates wound healing after fractionated
non-commercial use, distribution, radiation, suggesting hesperidin’s potential as a therapeutic paradigm in managing
and reproduction in any medium, irradiated wounds. Relevance for patients: The use of hesperidin is expected to
provided the original work is
properly cited. mitigate the complications of surgical wounds arising after radiotherapy of cancer
patients in a clinical setting.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Mice; Fractionated irradiation; Wound; Collagen; DNA; Glutathione
affiliations

Volume 11 Issue 1 (2025) 34 doi: 10.36922/jctr.24.00049