Page 102 - JCTR-11-4

Journal of Clinical and
Translational Research NADPH oxidase inhibition in a rodent stroke model

Appendix

Table A1. Glossary of antibodies used in immunofluorescence

Immunohistochemistry Endogenous block antibody Primary antibody Secondary antibody Fluorescent
staining combination (2‑h room temperature (4°C overnight incubation) (2‑h room temperature incubation) probe
incubation)
NeuN and DAPI - Rabbit anti-NeuN (1:500; Goat anti-rabbit IgG AlexaFluor 546 DAPI 10 mg/mL
AB177487, Abcam, UK) (1:500; A1101, Invitrogen, UK) (1:1000; D3571,
Invitrogen, UK)
1
NeuN and BrdU Goat anti-rabbit IgG (heavy and Rat anti-BrdU (BU1/75 [ICR ) Goat anti-rabbit IgG AlexaFluor 546 -
light chains) (1:300; 15450834, (1:200; AB6326, Abcam, UK); (1:500; A1101, Invitrogen, UK); Goat
Fisher, UK) Rabbit anti-NeuN (1:500; anti-rat IgG AlexaFluor 488 (1:400;
AB177487, Abcam, UK) AB150157, Abcam, UK)
CD31 - Rabbit anti-CD31 (1:200: Goat anti-rabbit IgG AlexaFluor 546 DAPI 10 mg/mL
AB222783; Abcam, UK) (1:800; A1101, Invitrogen, UK) (1:1000; D3571,
Invitrogen, UK)
Abbreviations: BrdU: 5-bromo-2’-deoxyuridine; DAPI: 4’,6-diamidino-2-phenylindole nuclear DNA stain; IgG: Immunoglobulin G; NeuN: Neuronal
nuclei; UK: United Kingdom.

Volume 11 Issue 4 (2025) 96 doi: 10.36922/jctr.25.00018