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Microbes & Immunity A novel anti-EphB2 monoclonal antibody for flow cytometry

Figure 3. Flow cytometry of endogenous EphB2-expressing cells using Eb Mab-12 and 2H9. LS174T cells were treated with 0.01 – 10 μg/mL of Eb Mab-12
2
2
or 2H9 conjugated with RB545 (red line). The Eb Mab-12 treated cells were further incubated with anti-mouse IgG conjugated with Alexa Fluor 488. The
2
fluorescence data were subsequently collected using the SA3800 Cell Analyzer. The black line represents the negative control (blocking buffer).
Abbreviation: IgG: Immunoglobulin G.
A

B

Figure 4. The binding affinity of Eb Mab-12 and 2H9. CHO/EphB2 (A) and LS174T (B) cells were suspended in serially diluted Eb Mab-12. The cells
2
2
were treated with anti-mouse IgG conjugated with Alexa Fluor 488. The cells were also suspended in serially diluted 2H9 conjugated with RB545. The
fluorescence data were subsequently collected using the BD FACSLyric, followed by the calculation of the K using GraphPad PRISM 6.
D
Abbreviation: IgG: Immunoglobulin G.

Volume 2 Issue 3 (2025) 173 doi: 10.36922/mi.5728