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Microbes & Immunity A novel anti-EphA8 monoclonal antibody
Appendix
A
B
Figure A1. Reproducibility of the binding affinity of Ea Mab-9
8
against EphA8-expressed cells. (A) CHO/EphA8 and (B)
LN229/EphA8 cells were incubated in 100 μL of serially diluted
Ea Mab-9 (10 μg/mL to 0.0006 μg/mL). Cells were then treated
8
with Alexa Fluor 488-conjugated anti-mouse IgG. Fluorescence
data were collected using the BD FACSLyric system, and K was
D
calculated using GraphPad PRISM 6 software.
Abbreviations: CHO: Chinese hamster ovary; GeoMean:
Geometric mean; K : Dissociation constant; EphA8:
D
Erythropoietin-producing hepatocellular receptor A8
Volume 2 Issue 4 (2025) 160 doi: 10.36922/MI025060010

