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Microbes & Immunity                                                  A novel anti-EphA8 monoclonal antibody



            Appendix


                                            A















                                            B

















                                           Figure A1. Reproducibility of the binding affinity of Ea Mab-9
                                                                              8
                                           against EphA8-expressed cells. (A) CHO/EphA8 and (B)
                                           LN229/EphA8 cells were incubated in 100 μL of serially diluted
                                           Ea Mab-9 (10 μg/mL to 0.0006 μg/mL). Cells were then treated
                                             8
                                           with Alexa Fluor 488-conjugated anti-mouse IgG. Fluorescence
                                           data were collected using the BD FACSLyric system, and K  was
                                                                                D
                                           calculated using GraphPad PRISM 6 software.
                                           Abbreviations: CHO: Chinese hamster ovary; GeoMean:
                                           Geometric mean; K : Dissociation constant; EphA8:
                                                        D
                                           Erythropoietin-producing hepatocellular receptor A8



























            Volume 2 Issue 4 (2025)                        160                           doi: 10.36922/MI025060010
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