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ORIGINAL RESEARCH ARTICLE
A trabeculae-like biomimetic bone-filling material

as a potential organoid for bone defect treatment

Fanjin Meng , Yang Yu , Jinshu Yu , Hong Chen , Yuwen Ma , Yonghao Chen , Yan Wang , Jingjing Jiang ,
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Xi He , Zhongyan Zhan , Pingping Yan , Jing Wen , Lijuan Zhang , Wei Chen , Airong Qian 5 , Xiaolan Guo ,
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Bing Yang *, Qi Liang *, Guangrong Wang *, Bin Guo *, Chong Yin *
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1 Department of Clinical Laboratory, Department of Oncology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan,
China
2 School of Laboratory Medicine, Translational Medicine Research Center, North Sichuan Medical College, Nanchong, Sichuan, China
3 School of Pharmacy, Tianjin Medical University, Tianjin, China
4 Xinjiang Huashidan Pharmaceutical Research Co., Ltd., Urumqi, Xinjiang Uygur Autonomous Region, China
5 Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences
and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone
Metabolism, School of Life Sciences, Northwestern Polytechnical University, 127 West Youyi Road, Xi’an, Shaanxi, China
6 Department of Public Health, International School, Krirk University, Bangkok, Thailand
*Corresponding authors: Bing Yang (yang.bing@krirk.ac.th); Qi Liang (liangqineijiang@126.com);
Guangrong Wang (13699669835@163.com); Bin Guo (guobin@nsmc.edu.cn); Chong Yin (yinchong42@nsmc.edu.cn)
Citation: Meng F, Yu Y, Yu J, et al. Abstract
A trabeculae-like biomimetic bone-
filling material as a potential organoid Bone-filling materials are critical tools for the treatment of bone defects. However,
for bone defect treatment. Organoid
Res. 2025;1(2):025040003. existing materials require improvements in tissue compatibility and drug-loading
doi: 10.36922/OR025040003 capacity. In this study, we designed and synthesized a novel trabeculae-like biomimetic
Received: September 30, 2024 bone-filling material (TBM) that mimics the composition and structure of natural
bone trabecular tissue. This TBM demonstrated high mechanical strength and
Revised: February 19, 2025
excellent biocompatibility. It effectively embedded osteogenic cells and potentially
Accepted: March 14, 2025 functioned as an organoid. We demonstrated that the TBM exhibited therapeutic
Published online: April 18, 2025 efficacy in treating various bone defects and fractures by filling defect regions and
enabling sustained release of small-molecule and nucleic acid drugs. Based on these
Copyright: © 2025 Author(s).
This is an Open-Access article findings, we propose TBM as a promising candidate for the treatment of bone defects
distributed under the terms of the and provide innovative insights for the development of bone-filling materials.
Creative Commons Attribution
License, permitting distribution, and
reproduction in any medium, which Keywords: Bone-filling material; Organoid; Bone defect; Tissue engineering
provided that the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with regard
to jurisdictional claims in published
maps and institutional affiliations.
1. Introduction treatment costs, severely impairing patients’ quality of life.
5
Consequently, the accurate and efficient treatment of bone
Bone defects can arise from fractures and various bone defects remains a major challenge in orthopedic research.
diseases, including bone tumors and osteomyelitis.
1,2
Critical-sized bone defects, in particular, cannot self-heal Bone-filling materials are essential tools for addressing
through intrinsic repair mechanisms. These defects bone defects, as they fill defect regions, provide mechanical
3,4
6-8
are characterized by prolonged rehabilitation periods, support, and promote bone formation. While autologous
high disability rates, and significant post-operative or allogeneic bone transplantation is considered the optimal

Volume 1 Issue 2 (2025) 1 doi: 10.36922/OR025040003