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particle characterization were successfully optimized. We have shown a device capable of a

95% sorting yield when singularizing microscaffolds at 100% precision when the sorting
solution was challenged with 50% defective constructs The precision of the system enabled not

only the automation of scale-up production of BBs within a variety of cell-culture wellplates,
but also allowed automated seeding of cell and in turn growth of single spheroids inside

microscaffolds with up to 1536 wells, ensuring a high throughput of building block formation

within a time window of 24 h.

Overall, the results of our study advanced the production of previously optimized hybrid

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spheroid microscaffolds as modular tissue buildng blocks to provide a robust basis for
production pipelines aiming at the third strategy for tissue engineering.

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