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Advanced Neurology Cytokine response to EV therapy in SCI
Appendix
IL‑2 491.42 236.89 VEGF 58.39 37.16 IL‑2* 955.11 368.14 VEGF 91.38 41.92 IL‑2 783.93 368.62 VEGF 76.27 29.55 IL‑2 510.74 322.05 VEGF 67.54 57.41
Table A1. Multiplex analysis of cytokine levels (pg/mL) in spinal cord homogenates (Th8) from control (SCI and SCI FM) and experimental (SCI FM+EVs5 and SCI
IL‑1b 88.45 22.71 TNF‑α 382.69 72.71 IL‑1b** 133.09 30.48 TNF‑α 443.78 66.61 IL‑1b 107.86 44.21 TNF‑α 401.07 155.25 IL‑1b 84.05 32.49 TNF‑α 363.19 136.28
IL‑1a 127.2 45.73 RANTES # 65.4 14.97 IL‑1a 115.34 18.01 RANTES 136.11 41.6 IL‑1a 92.7 44.66 RANTES 266.24 187.73 IL‑1a 67.43 49.62 RANTES 83.79 42.15
IL‑18 968.96 154.43 MIP‑3α 10.02 7.21 IL‑18* 1229.89 214.61 MIP‑3α* 23.32 9.43 IL‑18 1012.03 387.98 MIP‑3α 14.91 6.51 IL‑18 1085.37 193.01 MIP‑3α 10.27 8.65
IL‑13 83.6 67.25 MIP‑1α 76.32 28.41 IL‑13 173.94 67.58 MIP‑1α 65.68 31.63 IL‑13 112.00 59.22 MIP‑1α 70.47 33.13 IL‑13 89.95 80.9 MIP‑1α 58.95 34.64
IL‑12p70 67.52 45.12 MCP‑1 417.28 145.71 IL‑12p70 122.72 43.9 MCP‑1 425.51 160.03 IL‑12p70 96.98 56.27 MCP‑1 497.4 51.2 IL‑12p70 61.94 55.11 MCP‑1 413.48 73.81
IL‑10 160.71 147.75 M‑CSF 40.22 21.02 IL‑10** 383.05 155.00 M‑CSF* 68.17 19.09 IL‑10 267.65 129.06 M‑CSF 44.9 21.6 IL‑10 156.77 168.55 M‑CSF 43.21 33.27
IFN‑γ 198.42 167.21 IL‑17A 89.39 33.37 IFN‑γ* 454.60 182.50 IL‑17A** 123.77 20.43 IFN‑γ 299.97 161.49 IL‑17A 105.64 37.57 IFN‑γ 204.49 200.25 IL‑17A 75.34 47.59 Abbreviations: CSF: colony-stimulating factor; G-CSF: Granulocyte CSF; GM-CSF: Granulocyte–macrophage CSF; GRO/KC: Growth-related oncogene/keratinocyte-derived chemokine; IFN: Interferon; IL: Interleukin; M-CSF: macrophage CSF; MCP: Monocyte chemoattractant prot
GRO/KC 46.15 17.68 IL‑7 20.02 16.00 GRO/KC 41.97 11.67 IL‑7* 48.88 18.91 GRO/KC 39.15 14.59 IL‑7 33.44 14.30 GRO/KC 33.75 11.93 IL‑7 26.89 14.42 Notes: *p<0.05 compared to SCI group; **p<0.05 compared to SCI and SCI FM+EVs10 groups; # p<0.05 compared to SCI FM and SCI FM+EVs5 groups.
GM‑CSF 36.65 18.20 IL‑6 209.64 207.07 GM‑CSF* 60.38 15.97 IL‑6* 504.43 214.77 GM‑CSF 51.06 16.61 IL‑6 337.51 194.33 GM‑CSF 38.09 26.77 IL‑6 214.9 215.75
IL‑5 76.15 41.12 IL‑5** 142.57 44.97 IL‑5 115.92 41.8 IL‑5 74.14 48.99
G‑SCF 9.90 11.37 G‑CSF* 28.17 14.70 G‑SCF 15.73 8.8 G‑SCF 9.99 11.97
IL‑4 29.54 26.89 IL‑4 68.09 31.18 IL‑4 44.95 20.64 IL‑4 27.98 28.69
FM+EVs10) groups Mean value Standard deviation Mean value Standard deviation SCI FM Mean value Standard deviation SCI FM Mean value Standard deviation SCI FM+EVs5 Mean value Standard deviation SCI FM+EVs5 Mean value Standard deviation SCI FM+EVs10 Mean value Standard deviation SCI FM+EVs10 Mean value Standard deviation endothelial growth factor.
SCI
Volume 4 Issue 4 (2025) SCI 87 doi: 10.36922/AN025110022
