Page 235 - EJMO-9-2

Eurasian Journal of
Medicine and Oncology Quercetin effects in rats with liver injury

error of the mean, where the number of samples per group 3.3. Inflammatory markers
(n) equals seven. Multiple comparisons were calculated The group treated with paracetamol showed a significant
using analysis of variance, and p<0.05 denotes statistical increase in hepatic NF-κB and TNF-α levels compared
significance. to the control group (p<0.05). These findings suggest

3. Results that paracetamol administration induces a marked
inflammatory response in the liver. However, treatment
3.1. Liver enzyme levels with quercetin notably reduced the elevated levels of these
Administration of paracetamol resulted in a highly inflammatory markers, demonstrating its potential as an
significant increase in serum levels of GPT and GOT anti-inflammatory agent (p<0.05). This attenuation of the
compared to the control group (p<0.05). This proves inflammatory response highlights the therapeutic effects
to be hepatotoxic because an increase in these serum
transaminases is usually a result of liver damage. On the
other hand, pretreatment with quercetin showed a highly
considerable reduction in serum concentrations of both
GPT and GOT, indicating the possible hepatoprotective
effects of quercetin. These findings are statistically
significant (p<0.05), suggesting that quercetin protects
against paracetamol-induced hepatic injuries, as presented
in Figures 1 and 2.
3.2. Oxidative stress enzymes

Paracetamol administration caused a significant
increase in oxidative stress, as indicated by increased
MDA and reduced GSH levels. These changes led to
an increase in the lipid peroxidation process provoked
by toxicity due to paracetamol. On the other hand, Figure 2. Hepatic glutamate oxaloacetate transaminase (GOT) levels
quercetin administration resulted in a protective effect (U/L). The average hepatic GOT levels (U/L) among the four groups,
expressed as mean ± standard error of the mean, are significant at p<0.05.
against oxidative damage. Treatment with quercetin Notes: *refers to statistical significance between paracetamol versus
significantly decreased MDA levels and increased GSH the control group at p<0.05. refers to statistical significance between
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levels (p<0.05), indicating its potent antioxidant effect quercetin versus the paracetamol group at p<0.05.
(Figures 3 and 4).

Figure 1. Hepatic glutamate pyruvate transaminase (GPT) levels (U/L). Figure 3. Hepatic malondialdehyde (MDA) levels (nmol/mg). The
The average hepatic GPT levels (U/L) among the four groups, expressed average hepatic MDA levels (nmol/mg) among the four groups, expressed
as mean ± standard error of the mean, are significant at p<0.05. as mean ± standard error of the mean, are significant at p<0.05.
Notes: *refers to statistical significance between paracetamol versus Notes: *refers to statistical significance between paracetamol versus
the control group at p<0.05. refers to statistical significance between the control group at p<0.05. refers to statistical significance between
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quercetin versus the paracetamol group at p<0.05. quercetin versus the paracetamol group at p<0.05.
Volume 9 Issue 2 (2025) 227 doi: 10.36922/ejmo.7873