Page 261 - EJMO-9-2

Eurasian Journal of
Medicine and Oncology The genetics of chronic stress in cervical lesions

Figure 1. Flow diagram of sample processing
Abbreviations: PCR: Polymerase chain reaction; RFLP: Restriction fragment length polymorphism; SPSS: Statistical Package for Social Sciences;
VNTR: Variable number tandem repeats

Figure 2. Image of an agarose gel with a representative genotyping of
the SLC6A4 gene. Channels 1 – 7 show amplification products from
individuals under study. Channel 8 (CN) is the negative control, and
channel 9 (M) is the 100 base pair (bp) molecular weight marker (100 bp
DNA ladder). Note: 12/12 refers to homozygous normal (299 bp),
12/10 refers to heterozygous (299 bp and 265 bp), and 10/10 refers to
homozygous mutated (265 bp)

the seven genes and cervical lesions associated with HPV
infection. In this study, we used codominant, dominant, and Figure 3. Image of an agarose gel with a representative electrophoretic
genotyping of the ADRB2 gene. Channels 1 – 5 show the restriction
recessive genetic models to investigate allelic contribution products from individuals under study. Channel 1 (M) shows the 100
to the development of cervical lesions. As the inheritance base pair (bp) molecular weight marker (100 bp DNA ladder). Note: G/G
pattern for each variant is not known, codominant models indicates homozygous normal (291 bp), A/G indicates heterozygous (308
will provide an indication, while dominant/recessive and 291 bp), and A/A indicates homozygous mutated (308 bp)
models will refine the analysis. The allelic model is used
because of its ability to provide clear, focused insights into lesions. Ultimately, we want to understand how genetic
the relationship between genetic variants and cervical variants influence disease risk.

Volume 9 Issue 2 (2025) 253 doi: 10.36922/EJMO025100047