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Gene & Protein in Disease MPDZ regulates sperm motility

A B C D

E F G H

Figure 1. MPDZ-null males were subfertile. (A) Comparative analysis of litters sired by MPDZ-null males (K/K) and their wild-type (W/W) and
MPDZ-heterozygous (W/K) counterparts; 8 males per group, *p < 0.05 and **p < 0.001. Comparative analysis of the proportion of sperm with fast motility
(fast) (B), sperm with medium motility (medium) (C), sperm with slow motility (slow) (D), and sperm immotility (E) among the three genotypes. Comparative
analysis of the proportion of sperm progressive motility (F) and the average speed of spermatozoa among the three genotypes. Comparative analysis of the
proportion of sperm non-progressive motility (H). Eight male mice at 16 – 19 weeks of age from each genotype were included for statistical significance.
Abbreviation: MPDZ: Multiple PDZ domain protein.

Table 1. Binomial logistic regression analysis of the association between MPDZ expression and sperm motility of volunteers

Variables B SE Wals df Sig. HR 95% CI
Lower bound Upper bound
Ct M/G 0.130 0.206 0.398 1 0.528 1.601 0.760 1.707
Constant 35.550 33.947 1.097 1 0.295 0.000
Abbreviations: SE Standard error; HR: Hazard ratio; CI: Confidence interval.

fertility . Here, we found that the expression of the GC2 cells (Figure 3D-G). To further explore CatSper4
[11]
CatSper1–4 genes remarkably decreased at both the expression, we designed multiple pairs of primers and used
transcriptional and translational levels in MPDZ-null mouse testis cDNA as a positive control. We found that
spermatozoa (Figure 3A-C). These results indicated that CatSper4 did not express in GC2 cells (Figure S3). Thus,
MPDZ was involved in transcriptionally regulating the we concluded that MPDZ participated in the regulation of
expression of CatSper1–4 genes. The mouse MPDZ gene CatSper1/2 expression.
was overexpressed in GC2 cells, further supporting the
notion. In vitro, the expression of CatSper1/2 increased in 3.5. Transcription factor Stat3 played a role in the
MPDZ-overexpressing GC2 cells at both the transcriptional regulation of CatSper1/2 expression via MPDZ
and translational levels, but CatSper3 expression did Given that MPDZ generally acted as a typical scaffolding
not obviously change, and CatSper4 did not express in protein and was localized in the apical membrane [30,34] ,

Volume 2 Issue 2 (2023) 5 https://doi.org/10.36922/gpd.397