Page 40 - GPD-2-2

Gene & Protein in Disease MPDZ regulates sperm motility

A B C

D E F G

Figure 3. MPDZ regulated CatSper expression. (A) CatSper1–4 gene expressions in three genotypes were measured by qRT-PCR. (B) Changes of
CatSper1–4 genes at the translational level in spermatozoa were observed by performing western blots. (C) Quantification of the western blot results was
performed using ImageJ. Changes in CatSper1–4 expressions at the transcriptional levels (D) and translational levels (F) were detected using RT-PCR and
western blot. Quantification was performed using Image J (E, G).
Abbreviations: MPDZ: Multiple PDZ domain protein; qRT-PCR: Quantitative reverse transcription polymerase chain reaction.

A B C D

E F G H

Figure 4. Stat3 was essential for MPDZ-mediated upregulation of CatSper expression. (A) Changes in Stat3 (Y705) phosphorylation in vivo were detected
by western blot. (B) Image J was used to quantify the abundance of Stat3Y705 and Stat3 proteins. (C) The changes in Stat3 (Y705) phosphorylation and
Stat3 in 293T and GC2 cells overexpressing MPDZ were determined by western blot. (D) Quantification of Stat3 and Stat3Y705 was performed using
Image J. Inhibition of Stat3 (Y705) phosphorylation (E and F) and Stat3 expression by siRNA (G and H) decreased the abundance of CatSper1 and
CatSper2 proteins in GC2 cells, as shown by western blot.
Abbreviation: MPDZ: Multiple PDZ domain protein.

confirmed that Stat3 was responsible for the process 3.6. Molecular docking of MPDZ and Stat3
of MPDZ transcriptional regulating the expression of Computational docking methods have been widely
CatSper1/2. used to generate potential protein-protein interaction

Volume 2 Issue 2 (2023) 7 https://doi.org/10.36922/gpd.397