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Gene & Protein in Disease MPDZ regulates sperm motility

with bZIP domains is found to be involved in the activity Formal analysis: Dandan Wang, Tinglong Zhang, Xinying Ji,
of the CatSper1 promoter with the CRE elements identified Shaoping Ji, Jinyi Liu
in both human and murine CatSper1 promoters . Here, Investigation: Dandan Wang, Tinglong Zhang, Jianping Chen,
[51]
we find that Stat3, one of the STAT family of transcription Jingjing Huang, Jing Gu, Hao Liu, Yue Zhang
factors, is responsible for the transcription of CatSper1/2. Supervision: Dandan Wang, Xinying Ji, Shaoping Ji, Jinyi Liu
These different transcription factor families may play a Writing – original draft: All authors
regulatory role in the temporal expression of CatSper Writing – review & editing: All authors
genes to maintain intracellular Ca signal and sperm
2+
motility. However, how MPDZ regulates Stat3 needs Ethics approval and consent to participate
further investigation. The ethical approvals for animal and human subjects

5. Conclusion by the Ethics Committee for the Animal Research of
Army Medical University (Chongqing, China, approval
We show that MPDZ regulates sperm motility and fertility in ID: AWUMEC2020708) and the Ethics Committee of
mice and human sperm. The molecular mechanism revealed Wenzhou Medical University (WenZhou, China, approval
that sperm motility regulated by MPDZ is connected ID: AF/SC-08/1.0), respectively.
with the transcriptional regulation of the expression of
CatSper1/2 subunits by Stat3. MPDZ and Stat3 can create Consent for publication
a complex to phosphorylate Stat3 in Y705 (Figure 5). Our Not applicable.
studies enrich the understanding of regulatory mechanisms
of sperm motility and the function of MPDZ. Availability of data
Acknowledgments Additional data can be obtained from corresponding
author following formal request.
We are particularly grateful to the associate chief
technician Wuhua Ni (Reproductive Medicine Center, the References
First Affiliated Hospital of Wenzhou Medical University) 1. Hunault CC, Habbema JDF, Eijkemans MJC, et al., 2004, Two
for her kind help with analyzing and giving semen new prediction rules for spontaneous pregnancy leading to
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College of Preventive Medicine, Army Medical University) three previous models. Hum Reprod, 19: 2019–2026.
for her kindness in giving us the cDNA of human
spermatozoa; Lu Zhang, an assistant professor (Institute https://doi.org/10.1093/humrep/deh365
of Medical Bioinformatics, School of Basic Medicine, 2. Ghosh P, Mukherjee S, Bhoumik A, et al., 2018, A novel
Henan University) and Fengling Wang (Laboratory of epididymal quiescence factor inhibits sperm motility by
Cell Signal Transduction, Henan Provincial Engineering modulating NOS activity and intracellular NO-cGMP
Centre for Tumor Molecular Medicine, School of Basic pathway. J Cell Physiol, 233: 4345–4359.
Medicine, Henan University) for their kind help to analyze https://doi.org/10.1002/jcp.26275
the association between MPDZ expression and semen 3. Lishko PV, Kirichok Y, Ren D, et al., 2012, The control of
parameters of volunteers using SPSS 20.0 and R language. male fertility by spermatozoan ion channels. Annu Rev
Physiol, 74: 453–475.
Funding
https://doi.org/10.1146/annurev-physiol-020911-153258
This work was supported by grants from the Natural
Science Foundation Project of Chongqing CSTC of China 4. Achikanu C, Pendekanti V, Teague R, et al., 2018, Effects
of pH manipulation, CatSper stimulation and Ca -store
2+
(No. cstc2019jcyj-bshX0036 and cstc2020jcyj-bshX0036) mobilization on [Ca ]i and behaviour of human sperm.
2+
and the National Natural Science Foundation of China Hum Reprod, 33: 1802–1811.
(No. 81902711, 31902287 and 81670988).
https://doi.org/10.1093/humrep/dey280
Conflict of interest 5. Lievano A, Santi CM, Serrano CJ, et al., 1996, T-type Ca2+
There are no conflicts of interest to declare. channels and alpha1E expression in spermatogenic cells,
and their possible relevance to the sperm acrosome reaction.
Author contributions FEBS Lett, 388: 150–154.

Conceptualization: Dandan Wang, Tinglong Zhang, https://doi.org/10.1016/0014-5793(96)00515-7
Xinying Ji, Shaoping Ji, Jinyi Liu 6. Wennemuth G, Westenbroek RE, Xu T, et al., 2000,

Volume 2 Issue 2 (2023) 9 https://doi.org/10.36922/gpd.397