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Gene & Protein in Disease MPDZ regulates sperm motility

A

B C D

Figure 2. MPDZ was responsible for regulating the level of [Ca ] . (A) MPDZ was functionally relevant with Ca channel activity, store-operated calcium
2+
2+
i
channel activity, and inositol 1,4,5-trisphosphate (String database). (B) The relative fluorescence intensity of Fluo 4 in mouse spermatozoa imaged using a
fluorescence microscope. The relative Ca fluorescence intensity of mouse spermatozoa of three genotypes (C) and GC2 cells after MPDZ or vector control
2+
(VC) being overexpressed (D). Scale bar, 20 μm.
Abbreviation: MPDZ: Multiple PDZ domain protein.
we screened the transcription factor Stat3, as it could (Figure 4C and D). These results showed that MPDZ
bind to the promoter regions of CatSper1/2 using the promoted Stat3 phosphorylation.
JASPAR database (Table S2). To demonstrate the roles of To demonstrate the transcriptional regulatory role
Stat3 in the regulation of CatSper expression via MPDZ, of Stat3 in the CatSper genes, we inhibited Stat3 Y705
Stat3 expression was determined. We found that the using a selective inhibitor in MPDZ-overexpressing
abundance of Stat3 (Y705) phosphorylation, not Stat3 GC2 cells and found that CatSper1/2 expression
itself, significantly decreased in MPDZ-null mouse testes decreased significantly (Figure 4E and F). A similar
(Figure 4A and B). However, the content of Stat3 (Y705) observation was obtained after the inhibition of Stat3
phosphorylation, not Stat3 itself, increased considerably expression using siRNA in MPDZ-overexpressing
in GC2 and 293T cells when MPDZ was overexpressed GC2 cells (Figure 4G and H). These results further

Volume 2 Issue 2 (2023) 6 https://doi.org/10.36922/gpd.397