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Gene & Protein in Disease Dunaliella salina & myocardial ischemia-reperfusion injury

A B C

D
E

Figure 2. D. salina protected against MIRI in mice. (A and B) CK and LDH activity in coronary effluent. (C and D) Representative images of TTC staining
and averaged infarct size. (E) Representative pictures of H&E-stained cardiac tissue sections. Data were expressed as mean ± SEM, n = 8 per group.
## P < 0.01, compared with the control group; **P < 0.01, compared with the I/R group.
CK: Creatine kinase; D. salina: Dunaliella salina; H&E: Hematoxylin and eosin; I/R: Ischemia/Reperfusion; LDH: Lactate dehydrogenase; SEM: Standard
error of the mean; TTC: Triphenyltetrazolium chloride.

A B C D

E F G H

Figure 3. D. salina enhanced antioxidant activity after myocardial ischemia reperfusion in mice. (A) SOD activity and
(B) MDA content in myocardial homogenate. mRNA levels of (C) HO-1 and (D) NQO1. (E) Representative Western blot
images. (F) Quantity analysis of KEAP1, (G) NRF2, and (H) HO-1. Data were expressed as mean ± SEM, n = 8 per group.
#
## P < 0.01, P < 0.05, compare with the control group; **P < 0.01, *P < 0.05, compared with the I/R group.
D. salina: Dunaliella salina; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HO-1: Heme oxygenase-1; I/R: Ischemia/Reperfusion; KEAP1: Kelch-
like ECH-associated protein 1; MDA: Maleic dialdehyde; mRNA: messenger RNA; NRF2: Nuclear factor (erythroid-derived 2)-like 2; NQO1: NAD(P)H
quinone oxidoreductase 1; SEM: Standard error of the mean; SOD: Superoxide dismutase.

Volume 2 Issue 2 (2023) 5 https://doi.org/10.36922/gpd.387