Page 131 - GPD-3-3

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Genetic association of regulatory single

nucleotide variants in GSTA1 and SULT1A1 with
cigarette smoke-related lung cancer in an

eastern Indian population

1
Arpan Saha 1 , Madhurima Sinha , Susweta Roy 1 , Debmalya Sengupta 1 ,
3
2
Souradeep Banerjee 1 , Ritabrata Mitra , Abhijit Sarkar , Tamohan Chaudhuri ,
3
3
Gautam Bhattacharjee , Somsubhra Nath 4 , Susanta Roychoudhury 5 , and
1
Mainak Sengupta *
1 Department of Genetics, Faculty of Genetics, University of Calcutta, Kolkata, West Bengal, India
2 Department of Chest medicine, IPGME&R, Kolkata, West Bengal, India
3 Department of Radiation Oncology, Saroj Gupta Cancer Centre and Research Institute, Thakurpukur,
West Bengal, India
4 Institute of Health Sciences, Presidency University, Kolkata, West Bengal, India
5 Department of Molecular Genetics, CSIR-Indian Institute of Chemical Biology (TRUE Campus),
Kolkata, West Bengal, India

Abstract

The differential expression of genes involved in xenobiotic metabolism and DNA
*Corresponding author: repair has been implicated in the etiology of cigarette smoke-related lung cancer.
Mainak Sengupta Our research group has previously identified 22 gene expression-correlated DNAse
(msgntcs@caluniv.ac.in)
hypersensitive sequence-single nucleotide variants (SNVs) with regulatory potential
Citation: Saha A, Sinha M, Roy S, to modulate the transcription of seven xenobiotic metabolisms and seven DNA
et al. Genetic association of
regulatory single nucleotide variants repair genes, thereby contributing to individual susceptibility to lung cancer. In
in GSTA1 and SULT1A1 with this study, we evaluated the genetic association between lung cancer and two
cigarette smoke-related lung cancer regulatory SNVs (rSNVs) from the list of 22, namely, rs2207950 (A/G) and rs743590
in an eastern Indian population.
Gene Protein Dis. 2024;3(3):3928. (G/A), which modulate the expression of the xenobiotic metabolism genes GSTA1
doi: 10.36922/gpd.3928 and SULT1A1, respectively. A polymerase chain reaction-restriction fragment length
polymorphism-based association study was conducted among lung cancer patients
Received: June 13, 2024
Accepted: August 31, 2024 exposed to cigarette smoke and healthy smoker controls from an eastern Indian
Published Online: September 24, population. A risk genotype AA of GSTA1 rs2207950, with predictive potential,
2024 was significantly over-represented among the patients. This genotype is known to
Copyright: © 2024 Author(s). cause lower expression of GSTA1 compared to the other two genotypes, potentially
This is an Open-Access article leading to reduced efficiency in clearing cigarette smoke-associated xenobiotics,
distributed under the terms of the
Creative Commons Attribution thereby contributing to lung cancer development. Identifying such rSNVs in specific
License, permitting distribution, populations can contribute to the development of tailored therapies based on
and reproduction in any medium, precision medicine, as well as inform efficient legislation governing tobacco use and
provided the original work is
properly cited. genetic counseling.
Publisher’s Note: AccScience
Publishing remains neutral with Keywords: Cigarette smoke; Lung cancer; Xenobiotic metabolism; Regulatory single
regard to jurisdictional claims in
published maps and institutional nucleotide variant
affiliations.

Volume 3 Issue 3 (2024) 1 doi: 10.36922/gpd.3928