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Gene & Protein in Disease Comprehensive analysis of ZNF521/Zfp521

Table 1. Interacting proteins/miRNA of ZNF521/Zfp521
Protein name The binding regions Effects Reference
GLI1 and GLI2 N-terminal motif Promotion 3,10,14
miR-802 3’-UTR Inhibition 10
MLL fusion proteins 555 bp in 5’ ZNF521 Promotion 11
HDAC3 N-terminal motif Inhibition 9,15,24
NuRD complex N-terminal motif Inhibition 6,9,12,13,15,25
SIAH2 Unknown Inhibition 50,54
P300 ZNFs 1 – 8 Promotion 6,15,20-22
HDACs N-terminal motif Inhibition 9,13,15
GATA-1 N-terminal motif Inhibition 13,15,23,25
RUNX2 ZNFs 6 –10 and ZNFs 26-30 Inhibition 24
SOX3 Directly Control Unknown 21
E2A-PBX1 Unknown Promotion 38
EBF-1 ZNFs 24 –30 Unknown 13,36
GCACCCn ZNFs 2 – 8 Unknown 71
BMP2 ZNFs 9 –13 Inhibition 71
SMAD ZNFs 14 – 19 Unknown 23
miR-204-5p 3’-UTR Inhibition 26

Figure 2. Functions of ZNF521/Zfp521. The role of ZNF521/Zfp521 in hematopoiesis, fat, and neurodevelopment
Abbreviations: HSC: Hematopoietic stem cell; iNSCs: Induced neural stem cells.

through the control of RELA expression (Figure 2). expression of Fndc5. Forced expression of Zfp521
22
37
SUMOylation is a conserved dynamic protein post- enhances the neural turnover in ESCs. In contrast, the neural
translational modification process. In the regulation of differentiation in Zfp521-deficient mESCs is impaired.
21
hematopoiesis, Zfp521 plays a crucial role, which in turn Across its N-terminal zinc finger motif, Zfp521 synergizes
regulates erythroid differentiation through its direct with the P300 activator to induce the expression of
5,21
binding with GATA-1. 23,37 Furthermore, under stress the early neuroectoderm genes, including Sox1, Sox3,
conditions, the SUMOylation of Zfp521 contributes and Pax6, thereby stimulating neural differentiation 20,22
significantly to the reconstruction of erythroid. 29 (Figure 2). In addition, Zfp521 has the capability to convert
In addition, in mouse neural embryonic stem cells adult mouse brain-derived astrocytes into iNSCs (induced
(mESCs), over expression of Zfp521 increases the neural stem cells). Furthermore, human fibroblasts can
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Volume 3 Issue 3 (2024) 3 doi: 10.36922/gpd.3260