Page 139 - IJB-10-2

International

Journal of Bioprinting

REVIEW ARTICLE
Decellularized extracellular matrix for three-

dimensional bioprinted in vitro disease modeling

Mihyeon Bae 1,2† , Joeng Ju Kim 1,2† , Jongmin Kim , and Dong-Woo Cho *
1,2
1,2
1 Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH),
Pohang, Kyungbuk, Republic of Korea
2 POSTECH-Catholic Biomedical Engineering Institute, POSTECH, Pohang, Kyungbuk, Republic
of Korea
(This article belongs to the Special Issue: Fine-tuned Hydrogels for 3D Bioprinting)

Abstract
Precise in vitro models in tissue engineering have attracted the attention of researchers
seeking to understand physiological consequences from native tissues as well as the
mechanism of diseases in vitro. To construct delicate native tissue-like in vitro models, a
proper combination of biomimetic materials and a biofabrication strategy is required.
Conventional biomaterials, such as collagens, laminins, and synthetic polymers, have
been widely adapted in tissue recapitulation; however, they lack tissue specificity in
the context of biophysical properties and native-like extracellular matrix composition.
The lack of tissue specificity accounts for the pathophysiological discrepancy between
preclinical model and actual human patient. Thus, biomaterials should be improved for
attaining physiological similarity between disease models and patients. Additionally,
a biofabrication technique is essential for building mature cellular or tissue structures
† These authors contributed equally with a sophisticated bioassembly process. Among the biofabrication techniques,
to this work. bioprinting stands as a promising approach for constructing three-dimensional
*Corresponding author: (3D) cellular structures using specific cell types and biomaterials. Combining
Dong-Woo Cho multifunctional bioinks and bioprinting is expected to enhance tissue specificity with
(dwcho@postech.ac.kr)
regard to structural recapitulation. From this viewpoint, decellularized extracellular
Citation: Bae M, Kim JJ, Kim J, matrix (dECM) bioink has been increasingly used to achieve tissue specificity and
Cho D-W. Decellularized manufacturability in 3D bioprinting. Progress in this domain requires the clarification
extracellular matrix for three-
dimensional bioprinted in vitro of tissue-specific decellularization method and the development of a proper 3D
disease modeling. Int J Bioprint. bioprinting method, in conjunction with the improvement of the compatibility
2024;10(2):1970. between dECM and bioprinting. In this review, we introduce the production methods
doi: 10.36922/ijb.1970
and characteristics of dECM in the context of tissue specificity and examine state-of-
Received: October 6, 2023 the-art dECM-incorporated 3D-bioprinted in vitro models for disease investigation.
Accepted: November 3, 2023
Published Online: January 16, 2024 We also recommend a strategy for improving dECM for use in therapeutic studies
based on simulations of the pathophysiological microenvironment.
Copyright: © 2024 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution Keywords: Decellularized extracellular matrix; Bioink; 3D bioprinting; In vitro model;
License, permitting distribution, Biomaterial
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience 1. Introduction
Publishing remains neutral with
regard to jurisdictional claims in Modeling human tissues in vitro has contributed to advancements in biology,
published maps and institutional 1,2
affiliations. pharmacology, physiology, and medicine field. The rapid development of human tissue

Volume10 Issue 2 (2024) 131 doi: 10.36922/ijb.1970