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International Journal of Bioprinting Increased ECM stiffness enhances chemoresistance

Figure 1. Rheological measurement and Young’s modulus for different 3D-bioprinted models. (A) Temperature sweep tests showing storage modulus
(G′) and loss modulus (G″) with cooling rate of 5°C/min for 6% GelMA and 8% GelMA. (B) Time sweep tests showing G′ and G″ under 21.5°C for 6%
GelMA and 8% GelMA. (C) Optical images of 3D-printed models with different concentrations of GelMA. Scale bar: 500 μm. (D) Semi-quantitation of Pr
values of 3D-printed models with different concentrations of GelMA.

initial temperature of 37°C to the measured temperature 1.1. Figure 1D show that Pr values of 6% GelMA and 8%
of 21.5°C. To be specific, 8% GelMA had a relatively GelMA are 0.994±0.083 and 0.966±0.032, respectively.
higher modulus compared to 6% GelMA (Figure 1B). In 3.2. Construction of 3D-bioprinted ovarian cancer
our study, in order to evaluate the printability of bioinks model
semi-quantitatively, the Pr values of each concentration To simulate the TME in vivo, a 3D-bioprinted ovarian
of GelMA were calculated by Equation I. According cancer tumor model with a lattice structure was constructed
to previous study, 3D-bioprinted models will exhibit in vitro (Figure 2A). The 3D bioprinting technology allows
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satisfying morphology when Pr is in the range of 0.9 to for the quick generation of various fine structures with

Volume 10 Issue 3 (2024) 232 doi: 10.36922/ijb.1673