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International Journal of Bioprinting 3D-bioprinted hydrogel for pulp regeneration

Figure 9. DLP-based 3D-bioprinted DPGCs promote dental pulp regeneration in vivo. (A) Design and fabrication of the customized DPGC. (B) Schematic
diagram of TDM transplantation in immunodeficient mice. hDPSCs-laden DPGCs and bulk GelMA hydrogel constructs were combined with rat TDM,
and then subcutaneously transplanted into immunodeficient mice for 2 months. Empty TDM, as negative control, was directly transplanted into recipient
immunodeficient mice. (C) H&E staining images used for evaluating the newly regenerated tissue in each group. Dental pulp-like tissue containing more
microvessels (black arrow) and an odontoblast-like layer (yellow triangles) was regenerated in the DPGC group. (D) Immunohistological detection of
DSPP and CD31 protein. The black arrows denote that more microvessels were formed in the DPGC group. (E, F) Quantitative analysis of the DSPP- and
CD31-positive area for each group. **p < 0.01.

Volume 10 Issue 3 (2024) 315 doi: 10.36922/ijb.1790