Page 323 - IJB-10-4

International Journal of Bioprinting PCL/Fe3O4@ZIF-8 for infected bone repair

magnetic nanocomposite scaffolds promoted osteogenic Funding
differentiation by activating the Wnt/β-catenin signaling This study was supported by the National Natural Science
pathway of BMSCs. Specifically, the scaffolds upregulated Foundation of China (grant number: 81903249), the
the gene expression of β-catenin, Runx2, C-myc, C-jun, and Natural Science Basic Research Program of Shaanxi
Tcf-7 and the protein expression of β-catenin, RUNX2, province (grant number: 2024SF-YBXM-264, 2022JZ-
and p-GSK-3β. The addition of DKK1, an inhibitor of the 50, 2023-YBSF-291), and Xi’an Science and Technology
Wnt/β-catenin signaling pathway, can reverse these effects. Program (grant number: 22YXYJ0156). The funders had
These results indicated that the osteogenic effect of the no role in the design or outcome of this study.
PCL/Fe O @ZIF-8 scaffolds on BMSCs may be regulated
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by the Wnt/β-catenin signaling pathway. Conflict of interest
In this study, the 3D-printed PCL/10%Fe O @ZIF-
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8 composite scaffolds exhibited excellent antibacterial The authors declare no conflicts of interest.
ability while promoting new bone formation. Author contributions
Furthermore, histological analysis of the liver and kidney
did not show any substantial damage after 6 and 12 Conceptualization: Yun Xiao, Lei Tian, Jiankang He,
weeks of PCL/10%Fe O @ZIF-8 implantation (Figure Chunlin Zong
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S5 in Supplementary File). Thus, PCL/10%Fe O @ZIF-8 Formal analysis: Yun Xiao, Lei Tian, Chunlin Zong, Yi
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may not exert severe adverse effects, establishing itself Ding, Jun Qiu, Xiaonan Zhang, Yanzhen Zheng,
as a promising material with extensive applications in Mingchao Ding
the realm of bone repair. Therefore, PCL/Fe O @ZIF-8 Investigation: Yun Xiao, Lei Tian, Jiankang He, Chunlin
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scaffolds have ideal application potential in the repair of Zong, Jun Qiu, Chong Huang, Lu Zhao, Zihao Tang,
infected bone defects. However, the specific antibacterial Yuanli Chen, Yiwen Liu, Kezhen Zhao, Kai Guo, Li
and osteogenic mechanisms of the scaffolds were not Jing, Mingchao Ding
clarified in this study; therefore, further investigation is Writing – original draft: Yun Xiao, Chunlin Zong, Yi Ding,
needed. Moreover, further studies were needed to explore Jun Qiu
the degradation of composite scaffolds in vivo and the Writing – review & editing: Yun Xiao, Lei Tian, Jiankang
effects of degradation products on the microenvironment He, Chunlin Zong
of cell survival, such as pH value, osmotic pressure, and
metabolism of metal ions. Ethics approval and consent to participate

5. Conclusion All animal experiments were carried out in accordance
with the Chinese Animal Experiment Guidelines and
This study prepared 3D-printed PCL/Fe O @ZIF- approved by the Ethics Committee of the Fourth Military
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8 magnetic nanocomposite scaffolds fortified with Medical University (protocol number 20210972).
antibacterial and osteogenic capabilities to regenerate
bone in infected bone defects. The PCL/10%Fe O @ Consent for publication
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ZIF-8 scaffolds promoted the proliferation, adherence, Not applicable.
and osteoblast differentiation of BMSCs, indicating their
capacity to promote new bone formation. The prepared Availability of data
scaffolds also inhibited the proliferation and adhesion
of S. aureus and E. coli. These scaffolds also successfully All reported data are available from the corresponding
inhibited infection and promoted new bone formation authors upon reasonable request.
in infected bone defect, as demonstrated in animal
experiments. To the best of our knowledge, this study is References
the first to combine PCL and Fe O @ZIF-8 nanoparticles
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to prepare 3D-printed composite scaffolds featuring good 1. Yang Y, Chu L, Yang S, et al. Dual-functional 3D-printed
biocompatibility, antibacterial properties, and osteogenic composite scaffold for inhibiting bacterial infection and
promoting bone regeneration in infected bone defect
differentiation ability. The magnetic PCL/Fe O @ZIF- models. Acta Biomater. 2018;79:265-275.
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8 composite scaffolds have a huge clinical application doi: 10.1016/j.actbio.2018.08.015
potential in the repair of infected bone defects.
2. Wang W, Yeung KWK. Bone grafts and biomaterials
Acknowledgments substitutes for bone defect repair: a review. Bioact Mater.
2017;2(4):224-247.
None. doi: 10.1016/j.bioactmat.2017.05.007
Volume 10 Issue 4 (2024) 315 doi: 10.36922/ijb.2271