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International Journal of Bioprinting Pregabalin impact on 3D neuronal models

Figure 7. Neural activity changes in primary mouse embryonic cortical neurons (ECNs) before and after pregabalin exposure over 20 h. The recordings
were conducted on day 14 of cell culture in both 2D- and 3D-cultured ECNs. The graphs depict (A) the number of spikes, (B) the number of bursts, and
(C) the number of network bursts before and after pregabalin treatment. Data were derived from the activity recordings of two neuronal cell populations
with a minimum of three active electrodes. Data are presented as the mean ± standard error of the mean (SEM) and were analyzed using a t-test. * p < 0.05.
Abbreviation: PDL, poly-D-lysine.

testing and disease modeling. The hydrogel’s compatibility The following are the noteworthy findings of our
with high-throughput screening systems allows for the study: (i) pregabalin disrupts the mitochondrial electron
assessment of drug effects on complex, biologically relevant transport pathway, leading to metabolic failure; (ii)
3D neuronal cultures. This application is particularly pregabalin exposure does not affect the morphogenesis of
significant for studying neuroactive substances where the ECNs; (iii) in pregabalin-treated cultures, Olig2 and Zic1
3D microenvironment can influence drug responsiveness are prominently upregulated, whereas Dlx2, NhIh2, Otp,
in ways that traditional 2D cultures cannot. and Gad67 are significantly downregulated; (iv) pregabalin

Volume 10 Issue 4 (2024) 416 doi: 10.36922/ijb.3010