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International Journal of Bioprinting Pregabalin impact on 3D neuronal models

Figure 4. Morphological analysis of Tbr1-negative embryonic cortical neurons (ECNs) in response to pregabalin exposure. Effect of pregabalin on (A)
neurite length, (B) dominant neurite length, (C) number of branches, and (D) neurite number in Tbr1-negative ECNs in both groups. Representative
images of Tbr1-negative ECNs in the control group (E–H´) and pregabalin-treated group cultures (I–L´) do not display any significant differences in
morphology. F˝ and J˝ representing schematic illustration of neuronal morphology. Data are presented as the mean ± standard error of the mean (SEM); n
= 3 technical replicates and 4 biological replicates. Statistical analysis was performed using a t-test. Scale bars: 50 µm (E, F, G, H, I, J, K, and L); 10 µm (E´,
F´, F˝, G´, H´, I´, J´, J˝, K´, and L´). Red square represented the selected area; while orange arrows indicate the dominant neurite.

assessed for untreated cultures to compare the neural of bursts, burst frequency, durations of bursts, and inter-
activity between 2D- and 3D-cultured ECNs in PDL and burst intervals. Our data indicate that neurons were active
PDL/laminin scaffolds. The following neural matrices were in all three cell culture conditions. Compared to PDL, a
analyzed: number of spikes, mean firing rates, number significant increase in the average number of spikes, mean

Volume 10 Issue 4 (2024) 413 doi: 10.36922/ijb.3010