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International Journal of Bioprinting Pregabalin impact on 3D neuronal models

Figure 3. Morphological analysis of Tbr1-positive embryonic cortical neurons (ECNs) in response to pregabalin treatment. Effect of pregabalin on (A) neurite
length, (B) dominant neurite length, (C) number of branches, and (D) neurite number in Tbr1-positive ECNs in both groups. Microscopic images of control
(B, B´, C, C´, C˝, D, D´, E, and E´) and pregabalin-treated cultures (F, F´, J, J´, J˝, K, K´, L, and L´) demonstrate comparable morphology between the cultures.
Data are presented as the mean ± standard error of the mean (SEM); n = 3 technical replicates and four biological replicates. Statistical analysis was performed
using a t-test. Scale bars: 50 µm (B, C, D, E, F, J, K, and L); 10 µm (B´, C´, C˝, D´, E´, F´, J´, J˝, K´, and L´). Red arrows indicate the dominant neurite.

behavior of ECNs. Untreated ECN cultures were used as obstruction to alternating current flow, was measured
controls. PDL substrates, PDL/laminin-coated substrates, using the Maestro Pro MEA system (Axion Biosystems,
and IIZK-based scaffolds were used to establish the 2D and USA). The 2D- and 3D-cultured neurons on the PDL,
3D cultures. PDL/laminin, and IIZK-based scaffolds were viable on
To ensure the viability of neurons prior to recording day 14 of cell culture (Figure 6A and Video S1, Supporting
their spontaneous activity, the impedance, which indicates Information). Neural activity and oscillation were then

Volume 10 Issue 4 (2024) 412 doi: 10.36922/ijb.3010