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International Journal of Bioprinting Pregabalin impact on 3D neuronal models

Figure 8. High-throughput robotic 3D bioprinting and post-printing neuronal viability assessment and immunostaining of neuronal markers. (A)
Schematic representation of the 3D bioprinting process created with BioRender.com. (B) The in-house-developed robotic 3D bioprinter utilized in this
study is equipped with pumps for loading cells, peptides, and phosphate-buffered saline (PBS). (C) High-throughput 3D bioprinting of mouse embryonic
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cortical neuron cells within IIZK scaffolds. (D) 3D printing of acellular hydrogel-based bioink droplets, (E) and the printed construct shown as reference
outside of the 96-well plate. Live/dead staining of mouse cortical neurons (F) untreated with pregabalin and (H) treated cortical neurons, with live cells
appearing in green and dead cells in red highlighting the viability post-printing. Immunostaining for TUJ1 and TBR1 post-bioprinting in both (G)
untreated and (I) treated neurons is demonstrated, indicating successful neuronal marker expression. Scale bars: (F–I) 100 μm.

Volume 10 Issue 4 (2024) 417 doi: 10.36922/ijb.3010