Page 557 - IJB-10-4

International

Journal of Bioprinting

RESEARCH ARTICLE
Light-based and cost-effective bioprinting of

musculoskeletal GelMA constructs enriched with
mesoporous bioactive glass nanoparticles

Víctor Hugo Sánchez-Rodríguez 1† id , Juan Enrique Pérez-Cortez 2† id ,
Salvador Gallegos-Martínez 1 id , Cristina Chuck-Hernández 2,3 id ,
Ciro A. Rodriguez 2,4 id , Aldo R. Boccaccini , Elisa Vázquez-Lepe 2,4 id ,
5
Mario Moisés Alvarez 1† id , Grissel Trujillo-de Santiago * ,
1 id
*
and José Israel Martínez-López 2,4,6 id
1 Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey, Nuevo León, Mexico
2 Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, Nuevo León, Mexico
3 Institute for Obesity Research, Tecnologico de Monterrey, Monterrey, Nuevo León, Mexico
4 Laboratorio Nacional de Manufactura Aditiva MADiT, Apodaca, Nuevo León, Mexico
5
Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany
6 Centro de Investigación Numericalc, Monterrey, Nuevo León, Mexico

Abstract
† These authors contributed equally
to this work.
Bioprinting represents a promising technique for fabricating three-dimensional
*Corresponding authors: (3D) constructs with high-resolution and controlled architecture. However,
Grissel Trujillo-de Santiago
(grissel@tec.mx) many bioprinting technologies rely on expensive extrusion systems, which may
J. Israel Martínez-López compromise cell viability due to harsh processing conditions. This study presents the
(israel.mtz@tec.mx) fabrication and characterization of musculoskeletal tissue in gelatin methacryloyl
[GelMA]-based nanocomposite 3D constructs (comprising GelMA and mesoporous
Citation: Sánchez-Rodríguez VH,
Pérez-Cortez JE, bioactive glass nanoparticles [MBGNs]) using a cost-effective, light-based bioprinting
Gallegos-Martínez S, et al. (LBB) system. We demonstrated that our strategy can produce high-resolution
Light-based and cost-effective constructs (approximately 250 µm) while maintaining high cell viabilities (above
bioprinting of musculoskeletal
GelMA constructs enriched 85%) for extended periods (weeks of culture). Furthermore, the nanocomposite
with mesoporous bioactive constructs could facilitate the maturation of musculoskeletal tissue derived from
glass nanoparticles. C2C12 cells, as indicated by assessments of cell viability, elongation, and alignment
Int J Bioprint. 2024;10(4):1830.
doi: 10.36922/ijb.1830 over time. Our results suggested that the bioprinting approach outlined in this study
allows for precise control of architecture, while creating a conducive environment
Received: September 14, 2023
Accepted: May 20, 2024 for cell growth and tissue formation. These findings also highlighted the potential
Published Online: August 5, 2024 of the proposed LBB system to advance musculoskeletal tissue engineering for
Copyright: © 2024 Author(s). regenerative medicine applications.
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution Keywords: Light-based bioprinting; Mesoporous bioactive glass nanoparticles;
License, permitting distribution, Bioactive glass; Musculoskeletal tissue; GelMA; Bioprinting
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Skeletal muscle tissues constitute up to 50% of the total body weight and enable
1
2–4
affiliations. human mobility. Muscular disorders have been growing in prevalence, prompting
Volume 10 Issue 4 (2024) 549 doi: 10.36922/ijb.1830