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International Journal of Bioprinting Light-based muscle bioprinting with bioglass

Figure 1. A cost-effective light-based bioprinting (LBB) system. (A) Enhancement of a photo-crosslinkable bioink by the addition of mesoporous bioactive
nanoparticles (MBGNs). A blend of gelatin methacryloyl (GelMA), tartrazine, and MBGNs was used to induce C2C12 cell proliferation and alignment. (B)
LBB setup. (C) Retrofitted commercial printer components: modified vat and printing platform for bioinks. (D) Tensile strength testing. (E) Evaluation of
viability of C2C12 myoblasts. (F) Schematic of the skeletal muscle architecture. (G) Schematic of the bioprinted constructs and their expected time evolution.

Information) and Fourier-transform infrared spectroscopy this printer (Table 1) include layer height (LH), normal
(FTIR; Figure S3, Supporting Information). layer exposure time (LT), bottom layer exposure time
(BLT), and number of bottom layers (NBL).
2.2. Bioprinter
We converted an Anycubic Photon Mono 4K 3D printer The main frame of the printer comes with a plastic resin
(3840 × 2400 pixels) from Anycubic (Shenzhen, China) tank that can hold a volume of 300 mL and an aluminum
into an LBB system. The 3D printer was selected on grounds build plate that allows for the fabrication of objects with
of affordability, resolution, ease of use, and the ability to dimensions of up to 165 × 132 × 80 mm (height × width
change components. The printer costed approximately × length), which was replaced with a low-consumption
US$ 200, and it is widely available. Besides, the resulting resin tank and build plate in this study (Figure 1C). The
products from the 3D printer have a nominal horizontal retrofitting elements were designed using the computed-
resolution of 35 µm and a vertical resolution of 10 µm, aided design (CAD) software SolidWorks V2021 (Dassault
with a rated output power density of 3.75 mW/cm . The Systémes SolidWorks, Velizy-Villacoublay, France). The
2
equipment is controlled through a user interface with a reduced volume vat can operate with only 2.3 mL and is
2.8-in. touchscreen, and files are transferred to the printer composed of three main elements: circular frame, FEP
via a USB port using the Photon Workshop (V2.1.29) slicer film, and enclosure. The frame was built in aluminum
software. The printing parameters that can be adjusted for using subtractive manufacturing. The enclosure was

Volume 10 Issue 4 (2024) 552 doi: 10.36922/ijb.1830