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International Journal of Bioprinting Control nutrients to manipulate fungal growth

Figure 1. Ink formulation and 3D printing strategies for fungal-based ELMs. (A) Schematics showing the ink preparation with three levels of malt and
peptone content (low, medium, high) and the addition of liquid mycelium in the medium-content ink. These inks were then used in the multi-nozzle 3D
printer to create materials with local variations in nutrient content and mycelium. (B) Viscosity as a function of shear rate for the inks prepared with the
three nutrient levels. (C) Storage (G’) and loss (G’’) moduli as a function of shear stress for the inks prepared with the three nutrient levels. (D) Digital
images of the growth of G. lucidum and P. ostreatus in 3D-printed constructs over 7 days. Image contrast of all images was slightly enhanced for better
clarity. Scale bars: 20 mm. (E) Electron micrographs of the mycelium network of P. ostreatus and G. lucidum on the top and inside the macropores of
3D-printed constructs after 14 days of growth. Scale bars: 100 μm. For micrographs depicting sparse mycelium networks, red arrows are included to
indicate mycelium present.
Volume 10 Issue 5 (2024) 172 doi: 10.36922/ijb.3939