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International Journal of Bioprinting                             Control nutrients to manipulate fungal growth




            including bacteria and plant cells can also be incorporated   to dynamic behavior modulation.  Curr Opin Chem Biol.
            and investigated, provided that the printability of the ink is   2022;70:102188.
            not affected by the incorporation of such materials. Fungal      doi: 10.1016/j.cbpa.2022.102188
            behavior in complex architectures manufactured via 3D   3.   Lantada AD, Korvink JG, Islam M. Taxonomy for engineered
            printing can also be another potential area inspired by this   living materials. Cell Rep Phys Sci. 2022;3(4):100807.
            study, opening the way for the exploration of 3D-printed      doi: 10.1016/j.xcrp.2022.100807
            biodegradable and living products.                 4.   Vandelook S, Elsacker E, Van Wylick A, De Laet L, Peeters
                                                                  E. Current state and future prospects of pure mycelium
            Acknowledgements                                      materials. Fungal Biol Biotechnol. 2021;8(1):20.
                                                                  doi: 10.1186/s40694-021-00128-1
            None.
                                                               5.   Jones M, Gandia A, John S, Bismarck A. Leather-like material
            Funding                                               biofabrication using fungi. Nat Sustain. 2021;4(1):9-16.
                                                                  doi: 10.1038/s41893-020-00606-1
            The authors acknowledge funding from the National
            Research Foundation of Singapore and ETH Zurich,   6.   Elsacker E, Zhang M, Dade-Robertson M. Fungal
                                                                  engineered living materials: the viability of pure mycelium
            Switzerland, with the grant Future Cities Laboratory   materials with self-healing functionalities. Adv Funct Mater.
            Global, Module A4: Mycelium digitalization.           2023;33(29):2301875.
                                                                  doi: 10.1002/adfm.202301875
            Conflict of interest
                                                               7.   McBee RM, Lucht M, Mukhitov N, et al. Engineering living
            The authors declare they have no competing interests.   and regenerative fungal–bacterial biocomposite structures.
                                                                  Nat Mater. 2022;21(4):471-478.
            Author contributions                                  doi: 10.1038/s41563-021-01123-y
            Conceptualization: Jia Heng Teoh, Hortense Le Ferrand  8.   Gantenbein S, Colucci E, Käch J, et al. Three-dimensional
            Formal analysis: Jia Heng Teoh                        printing of mycelium hydrogels into living complex
                                                                  materials. Nat Mater. 2023;22(1):128-134.
            Funding acquisition: Hortense Le Ferrand              doi: 10.1038/s41563-022-01429-5
            Investigation: Jia Heng Teoh
            Methodology: Jia Heng Teoh, Eugene Soh             9.   Brand A, Gow NA. Mechanisms of hypha orientation of
            Supervision: Hortense Le Ferrand                      fungi. Curr Opin Microbiol. 2009;12(4):350-357.
            Visualization: Jia Heng Teoh, Hortense Le Ferrand     doi: 10.1016/j.mib.2009.05.007
            Writing – original draft: Jia Heng Teoh            10.  Soh E, Le Ferrand H. Woodpile structural designs to increase
            Writing – review & editing: All authors               the stiffness of mycelium-bound composites. Mater Design.
                                                                  2023;225:111530.
            Ethics approval and consent to participate            doi: 10.1016/j.matdes.2022.111530
            Not applicable.                                    11.  Murphy SV, Atala A. 3D bioprinting of tissues and organs.
                                                                  Nat Biotechnol. 2014;32(8):773-785.
            Consent for publication                               doi: 10.1038/nbt.2958
                                                               12.  Schaffner M, Rühs PA, Coulter F, Kilcher S, Studart AR. 3D
            Not applicable.                                       printing of bacteria into functional complex materials. Sci
                                                                  Adv. 2017;3(12):eaao6804.
            Availability of data                                  doi: 10.1126/sciadv.aao6804

            Additional data are available in the Supplementary File.   13.  Teoh JH, Mozhi A, Sunil V, Tay SM, Fuh J, Wang CH. 3D
            Data  are  available  from  the  corresponding  author  upon   printing personalized, photocrosslinkable hydrogel wound
            reasonable request.                                   dressings for the treatment of thermal burns.  Adv Funct
                                                                  Mater. 2021;31(48):2105932.
            References                                            doi: 10.1002/adfm.202105932
                                                               14.  Skylar-Scott MA, Mueller J, Visser CW, Lewis JA. Voxelated
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